HPLC methods for profiling and estimation of withanolide in Withania have been developed to study seasonal, geographical and genetic variation in WithaferinA content and identify chemotypes. Highest withanolide content was observed during the vegetative phase and slight decrease was observed during reproductive phase. However, the content drastically reduced at the maturity. No significant effect of location was observed on the withanolide content when same genotypes were grown at two diverse locations. WithaferinA content in Indian population of Withania somnifera varied from 0.22 to 3.38 % with a mean value of 1.099 in the leaves during vegetative stage.Whereas during maturity the content ranged from 0.0056 to 1.99 % with a mean value of 0.5942 %. In the roots, WithaferinA content ranged from 0.0028 to 0.75 % with a mean value of 0.1242 during vegetative phase and 0.0008 to 0.1489 with a mean value of 0.00213 % at maturity of the plant. The population segregated into two chemotypes identified as chemotypeI and as an intermediate of ChemotypeI and ChemotypeII.

中文翻译：

---

WithWithania somnifera（Linn。）Dunal的HPLC分析揭示了印度种质的两种化学型以及Withaferin A的季节性和遗传变异

已经开发出了用于在Withania中分析和评估Withanolide的HPLC方法，以研究WithaferinA含量的季节性，地理和遗传变异并鉴定化学型。在营养阶段观察到最高的山梨醇含量，在生殖阶段观察到轻微的下降。然而，该含量在成熟时急剧降低。当相同的基因型在两个不同的位置生长时，未观察到位置对withanolide含量的影响。营养期印度叶子枯萎病种群中WithaferinA含量在0.22％至3.38％之间，平均值为1.099;而在成熟期，其含量在0.0056％至1.99％之间，平均值为0.5942％。在根中，WithaferinA含量范围为0.0028至0.75％，平均值为0。营养期为1242，成熟期为0.0008至0.1489，平均值为0.00213％。种群分为两种化学型，分别是化学型I和化学型I与化学型II的中间产物。