Novel fluorescent labels with high photostability and high biocompatibility are required for microbiological imaging and detection. Here, we present a green fluorescent polymer chain (GFPC), designed to be nontoxic and water-soluble, for multicolor bioimaging and real-time bacterial viability determination. The copolymer is synthesized using a straightforward one-pot reversible addition–fragmentation chain-transfer (RAFT) polymerization technique. We show that GFPC does not influence bacterial growth and is stable for several hours in a complex growth medium and in the presence of bacteria. GFPC allows the labeling of the bacterial cytoplasm for multicolor bacterial bioimaging applications. It can be used in combination with propidium iodide (PI) to develop a rapid and reliable protocol to distinguish and quantify, in real time, by flow cytometry, live and dead bacteria.

中文翻译：

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用于细菌生物成像和生存力检测的荧光共聚物。

微生物成像和检测需要具有高光稳定性和高生物相容性的新型荧光标记。在这里，我们介绍了绿色荧光聚合物链（GFPC），该链被设计为无毒且水溶性的，可用于多色生物成像和实时细菌生存力测定。该共聚物是使用简单的一锅可逆加成-断裂链转移（RAFT）聚合技术合成的。我们表明，GFPC不会影响细菌的生长，并且在复杂的生长培养基中和存在细菌的情况下稳定数小时。GFPC允许为多色细菌生物成像应用标记细菌细胞质。它可以与碘化丙啶（PI）结合使用，以开发一种快速可靠的方案，以通过流式细胞仪实时区分和定量，