Abstract Lung squamous cell carcinoma (LUSC) is highly malignant with poor prognosis, causing prevalent mortality worldwide. Early-stage lung cancer can be distinguished through reliable early detection to urge timely treatment. Non-coding RNAs (ncRNAs) might play such a vital role in tumor initiation, progression and metastasis that they are becoming an important biomarker in cancer screening. To identify the relation between ncRNAs and the early-stage of LUSC, we analyzed the data from the Cancer Genome Atlas (TCGA) database. We identified 28 microRNAs (miRNAs) and 32 long non-coding RNA (lncRNA) that were specifically expressed in early-stage LUSC, and the relationship was shown by the lncRNA-miRNA-mRNA competing endogenous RNA (ceRNA) network. The result derived from Kaplan–Meier survival curves analysis showed that two miRNAs (hsa-miR-22-3p and hsa-miR-552-3p) and a lncRNA (FLJ36000) were negatively correlated with overall survival, but two miRNAs (hsa-miR-27b-5p and hsa-miR-340-5p) and five lncRNAs (CHK8-CPT1B, LINC00167, S NHG15, TTC28-AS1 and WASH7P) were positively associated. Our study provides novel insight for better understanding of early-stage LUSC and facilitates the identification of potential biomarkers for diagnosis and prognosis of early-stage LUSC.

中文翻译：

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基于 TCGA 数据集分析的早期鳞状细胞癌中 microRNA 和 lncRNA 的表征

摘要 肺鳞状细胞癌（LUSC）恶性程度高，预后差，导致全球普遍死亡。早期肺癌可以通过可靠的早期发现来区分，以促使及时治疗。非编码 RNA (ncRNA) 可能在肿瘤的发生、进展和转移中发挥着至关重要的作用，以至于它们正在成为癌症筛查中的重要生物标志物。为了确定 ncRNA 与早期 LUSC 之间的关系，我们分析了来自癌症基因组图谱 (TCGA) 数据库的数据。我们鉴定了在早期 LUSC 中特异性表达的 28 个 microRNA (miRNA) 和 32 个长链非编码 RNA (lncRNA)，lncRNA-miRNA-mRNA 竞争性内源 RNA (ceRNA) 网络显示了这种关系。Kaplan-Meier生存曲线分析结果表明，两种miRNA（hsa-miR-22-3p和hsa-miR-552-3p）和一种lncRNA（FLJ36000）与总生存率呈负相关，但两种miRNA（hsa- miR-27b-5p 和 hsa-miR-340-5p）和五种 lncRNA（CHK8-CPT1B、LINC00167、S NHG15、TTC28-AS1 和 WASH7P）呈正相关。我们的研究为更好地了解早期 LUSC 提供了新的见解，并有助于识别用于早期 LUSC 诊断和预后的潜在生物标志物。