ABSTRACT

In the wake of the reproducibility crisis and numerous discussions on how commercially available antibodies as research tool contribute to it, The Antibody Society developed a series of 10 webinars to address the issues involved. The webinars were delivered by speakers with both academic and commercial backgrounds. This report highlights the problems, and offers solutions to help the scientific community appropriately identify the right antibodies and to validate them for their research and development projects. Despite the various solutions proposed here, they must be applied on a case-by-case basis. Each antibody must be verified based on the content of the product sheet, and subsequently through experimentation to confirm integrity, specificity and selectivity. Verification needs to focus on the precise application and tissue/cell type for which the antibody will be used, and all verification data must be reported openly. The various approaches discussed here all have caveats, so a combination of solutions must be considered.

摘要

在可再现性危机以及关于市售抗体作为研究工具如何发挥作用的众多讨论之后，抗体协会开发了一系列10场网络研讨会来解决所涉及的问题。网络研讨会由具有学术和商业背景的发言人进行。该报告重点介绍了问题，并提供了解决方案，以帮助科学界适当地识别正确的抗体，并对其研发项目进行验证。尽管此处提出了各种解决方案，但必须逐案应用。必须根据产品说明书的内容并随后通过实验确认每种抗体的完整性，特异性和选择性。验证需要集中在将要使用抗体的精确应用和组织/细胞类型上，所有验证数据必须公开报告。这里讨论的各种方法都有警告，因此必须考虑多种解决方案。