ABSTRACT Aflatoxin M1 (AFM1) in milk is a problem of great concern. Current methods of detection require large instruments and need specific test sites. Therefore, it is necessary to establish a fast, convenient, and accurate detection method for AFM1. We established a system based on fluorescent microspheres containing a Eu3+ chelate named AFM1-POCT. These components comprised the AFM1-POCT kits. After refrigeration at 4°C for 12 months, the intra and inter assay coefficients of variability (CVs) for the kits were 4% and 5%, respectively. AFM1-POCT compared well with ultra-high-performance liquid chromatography (UHPLC) determination in the range 0.0121–2 μg/kg (paired samples test, P > 0.05). Aflatoxin B1 and G1 do not react with aflatoxin M1. By using the AFM1-POCT method, the detection time is shortened to 5 min, the accuracy is comparable to that of UHPLC, and convenience and range of application are improved.

中文翻译：

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快速、超灵敏测定牛奶中黄曲霉毒素 M1 的方法

摘要 牛奶中的黄曲霉毒素 M1 (AFM1) 是一个备受关注的问题。当前的检测方法需要大型仪器并需要特定的测试地点。因此，有必要建立一种快速、方便、准确的AFM1检测方法。我们建立了一个基于荧光微球体的系统，该微球体含有一种名为 AFM1-POCT 的 Eu3+ 螯合物。这些组件包括 AFM1-POCT 套件。在 4°C 下冷藏 12 个月后，试剂盒的测定内和测定间变异系数 (CV) 分别为 4% 和 5%。AFM1-POCT 在 0.0121–2 μg/kg 范围内与超高效液相色谱 (UHPLC) 测定结果相得益彰（配对样品测试，P > 0.05）。黄曲霉毒素 B1 和 G1 不与黄曲霉毒素 M1 发生反应。通过使用AFM1-POCT方法，检测时间缩短至5分钟，