This study analyzed the antimicrobial, cytotoxic, and antioxidant properties of Cnidiumofficinale (CO) extracts to confirm their antimicrobial activity toward oral microorganisms. The control group contained 0 μg/mL of CO, and the experimental groups contained 50, 100, 150, and 200 μg/mL of CO. To confirm the antibacterial activity of CO extracts against microorganisms in the oral cavity, an inhibition zone test, a colony-forming unit (CFU) analysis, an optical density (OD) evaluation, and a SEM (scanning electron microscopy) analysis were performed. A cytotoxicity test was also conducted to determine cell viability, and the contents of flavonoids and polyphenols were measured to analyze the extract components. In the control group, the growth inhibition zone increased, while the CFU and OD values decreased (p < 0.05). The SEM analysis confirmed that the number of microorganisms for both the microbes decreased. The cell viability was more than 80% in both the control and experimental groups, excluding the 200 μg/mL sample. The flavonoid and polyphenol contents in the experimental groups showed higher values than those of the control group. Therefore, the CO extract showed considerable antimicrobial activity toward both Streptococcus mutans and Candida albicans, suggesting that it may be used as a natural antimicrobial agent for dental applications.

中文翻译：

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虎杖提取物的抗微生物，细胞毒性和抗氧化活性分析。

这项研究分析了Cndium officinale的抗菌，细胞毒性和抗氧化特性（CO）提取物，以确认其对口腔微生物的抗菌活性。对照组的CO含量为0μg/ mL，实验组的CO含量为50、100、150和200μg/ mL。为确定CO提取物对口腔微生物的抗菌活性，需进行抑制区测试，进行菌落形成单位（CFU）分析，光密度（OD）评估和SEM（扫描电子显微镜）分析。还进行了细胞毒性测试以确定细胞活力，并测量了类黄酮和多酚的含量以分析提取物成分。在对照组中，生长抑制区增加，而CFU和OD值降低（p<0.05）。SEM分析证实两种微生物的微生物数量均减少。除200μg/ mL样品外，对照组和实验组的细胞活力均超过80％。实验组中的类黄酮和多酚含量高于对照组。因此，CO提取物对变形链球菌和白色念珠菌均显示出显着的抗菌活性，表明它可用作牙科应用的天然抗菌剂。