The transcriptome profile and differential gene expression in telogen and late anagen microdissected hair follicles and the interfollicular epidermis of healthy dogs was investigated by using RNAseq. The genes with the highest expression levels in each group were identified and genes known from studies in other species to be associated with structure and function of hair follicles and epidermis were evaluated. Transcriptome profiling revealed that late anagen follicles expressed mainly keratins and telogen follicles expressed GSN and KRT15. The interfollicular epidermis expressed predominately genes encoding for proteins associated with differentiation. All sample groups express genes encoding for proteins involved in cellular growth and signal transduction. The expression pattern of skin-associated genes in dogs is similar to humans. Differences in expression compared to mice and humans include BMP2 expression mainly in telogen and high KRT17 expression in the interfollicular epidermis of dogs. Our data provide the basis for the investigation of the structure and function of canine skin or skin disease and support the use of dogs as a model for human cutaneous disease by assigning gene expression to specific tissue states.

中文翻译：

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犬显微切割的生长期和休止期毛囊和毛囊间表皮中的转录组分析和差异基因表达

通过使用 RNAseq 研究了休止期和晚期生长期显微解剖的毛囊和健康狗的毛囊间表皮中的转录组谱和差异基因表达。确定了每组中表达水平最高的基因，并评估了从其他物种研究中已知的与毛囊和表皮的结构和功能相关的基因。转录组分析显示晚期毛囊主要表达角蛋白，休止期毛囊表达 GSN 和 KRT15。毛囊间表皮主要表达编码与分化相关的蛋白质的基因。所有样品组都表达编码参与细胞生长和信号转导的蛋白质的基因。狗的皮肤相关基因的表达模式与人类相似。与小鼠和人类相比，表达的差异包括主要在休止期的 BMP2 表达和狗的毛囊间表皮中的高 KRT17 表达。我们的数据为研究犬皮肤或皮肤病的结构和功能提供了基础，并通过将基因表达分配给特定组织状态来支持将狗用作人类皮肤病的模型。