Circular RNAs (circRNAs), which are considered a large class of endogenous noncoding RNAs, function as regulators in various biological procedures. In this study, the function and molecular mechanisms of circRNA8220 in goat mammary epithelial cells (GMECs) were explored. CircRNA8220 could spong miR-8516 and block the function of miR-8516 by binding to the target site of miR-8516 a negative feedback relationship existed between circRNA8220 and miR-8516. Stanniocalcin 2 (STC2) was a target gene of miR-8516. circRNA8220 could up-regulate the expression of STC2 by sponging miR-8516 in GMECs. circRNA8220/miR-8516/STC2 could promote proliferation and enhance the synthesis of β-casein and triglycerides (TG) via Ras/MEK/ERK and PI3K/AKT/mTOR signaling pathways, respectively.

中文翻译：

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CircRNA8220通过Mis-8516海绵来调节山羊乳腺上皮细胞中Ras / MEK / ERK和PI3K / AKT / mTOR途径的细胞活力和牛奶合成。

环状RNA（circRNA）被认为是一大类内源性非编码RNA，它们在各种生物学程序中起调节剂的作用。在这项研究中，探讨了circRNA8220在山羊乳腺上皮细胞（GMECs）中的功能和分子机制。CircRNA8220可以与miR-8516的靶位点结合，从而刺激miR-8516并阻断miR-8516的功能，circRNA8220与miR-8516之间存在负反馈关系。斯坦钙蛋白2（STC2）是miR-8516的靶基因。circRNA8220可以通过在GMECs中掺入miR-8516来上调STC2的表达。circRNA8220 / miR-8516 / STC2 可以通过Ras / MEK / ERK和PI3K / AKT / mTOR信号通路促进增殖并增强β-酪蛋白和甘油三酸酯（TG）的合成。