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Effect of using a nitrogen atmosphere on enzyme hydrolysis at high corn stover loadings in an agitated reactor.
Biotechnology Progress ( IF 2.9 ) Pub Date : 2020-08-03 , DOI: 10.1002/btpr.3059
Antonio Carlos Freitas Dos Santos 1, 2 , Eduardo Ximenes 1, 2 , David N Thompson 3 , Allison E Ray 3 , Ryan Szeto 4 , Kendra Erk 4 , Bruce S Dien 5 , Michael R Ladisch 1, 2
Affiliation  

A comprehensive review of the literature shows that enzyme hydrolysis efficiency decreases with increased solids loadings at constant enzyme:cellulose ratios for pretreated lignocellulosic substrates. In seeking a mechanistic explanation for this phenomenon, we found that a nitrogen atmosphere enhances enzyme hydrolysis and minimizes the decrease in glucose yields as solids loadings are increased in an agitated bioreactor. For liquid hot water pretreated corn stover, at solids loadings of both 100 and 200 g/L and hydrolyzed for 72 hr in a 1 L bioreactor at pH 5.0 with 3.6 mg protein per g biomass, glucose yields were 55% in a nitrogen atmosphere versus 45% in air with agitation and about 34% without agitation. While mixing promotes biomass/enzyme contact and disperses sugars released during hydrolysis that would otherwise cause product inhibition, nitrogen gas displaces air, avoiding deactivation of cellulases by oxygen. The nitrogen effect points to a facile approach of enhancing hydrolysis at high solids loadings.

中文翻译:

在搅拌反应器中使用氮气氛对高玉米秸秆负载下酶水解的影响。

对文献的全面回顾表明,对于预处理的木质纤维素底物,酶水解效率随着酶: 纤维素比率恒定的情况下固体负载量的增加而降低。在寻求对这种现象的机械解释时,我们发现,随着搅拌生物反应器中固体负载的增加,氮气气氛增强了酶水解并使葡萄糖产量的下降最小化。对于液态热水预处理的玉米秸秆,在 100 和 200 g/L 的固体负载下,并在 pH 5.0 的 1 L 生物反应器中水解 72 小时,每克生物质含 3.6 mg 蛋白质,与氮气氛相比,葡萄糖产率为 55% 45% 在空气中搅拌,约 34% 不搅拌。虽然混合促进生物质/酶接触并分散水解过程中释放的糖,否则会导致产品抑制,氮气置换空气,避免纤维素酶被氧气灭活。氮效应表明在高固体负载下增强水解的简便方法。
更新日期:2020-08-03
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