Two monoclonal antibodies (mAbs) specific to Salmonella were produced from a mouse immunized with a combination of heat‐killed whole‐cell and formalin‐fixed Salmonella enterica serovar Enteritidis (S. Enteritidis) as the antigens. The mAb SE‐13D bound specifically only to S. Enteritidis, while mAb Sal‐06G bound to various members of the Salmonella serogroups B, C, D, E, and I. Neither mAb demonstrated any cross‐reactivity to other bacteria. The two mAbs belong to the IgM class and IgG2a subclass, respectively. Using a dot‐blot assay, the detection sensitivity limits of mAbs SE‐13D and Sal‐06G were 10⁷ and 10⁶ cfu/ml, respectively. However, the detection sensitivity of both mAbs could be improved to 10⁴ or 1 cfu/ml after pre‐enriching the bacteria in tryptic soy broth (TSB) or chicken homogenate in TSB for 3 or 12 hr, respectively. Therefore, mAbs SE‐13D and Sal‐06G can be used as simple, rapid, specific, and effective immunological tools to directly detect and differentiate S. Enteritidis and an assortment of Salmonella serovars from other bacteria in complex crude samples such as poultry products, foods, and medical samples without the need for bacterial isolation and the conventional characterizations required by biochemical tests.

中文翻译：

---

使用斑点印迹法开发用于快速检测和鉴定食品样品中肠炎沙门氏菌的单克隆抗体

用热灭活的全细胞和福尔马林固定的肠炎沙门氏菌肠炎沙门氏菌肠炎沙门氏菌（S. Enteritidis）组合免疫，产生了两种沙门氏菌特异的单克隆抗体（mAb）。的mAb，SE-13D仅特异性结合到S.肠炎，而单克隆抗体萨尔-06G结合到的各种构件沙门氏菌血清群B，C，d，E，和I既不单抗表现出任何交叉反应性的其它细菌。这两个单克隆抗体分别属于IgM类和IgG2a亚类。使用斑点印迹分析，mAb SE-13D和Sal-06G的检测灵敏度极限为10 ⁷和10 ⁶cfu / ml。但是，将细菌预富含胰蛋白酶大豆肉汤（TSB）或鸡匀浆中的细菌3小时或12小时后，两种mAb的检测灵敏度均可提高到10 ⁴或1 cfu / ml。因此，单克隆抗体SE-13D和Sal-06G可以被用作简单，快速，特异性的，和有效的免疫学工具直接检测和区分S.肠炎和的分类沙门氏菌从复杂粗样品中其它细菌如家禽产品血清型，食品和医疗样品，而无需细菌分离和生化测试所需的常规表征。