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Antimicrobial activity of bacteriocin‐producing Carnobacterium spp. isolated from healthy Patagonian trout and their potential for use in aquaculture
Aquaculture Research ( IF 2 ) Pub Date : 2020-08-04 , DOI: 10.1111/are.14806
Marisa E. Garcés 1 , Nelda L. Olivera 1 , Melania Fernández 2, 3 , Carla Riva Rossi 4 , Cynthia Sequeiros 2
Affiliation  

In this study, bacteria from the intestines of Patagonian trout displaying fish pathogen antimicrobial activity were isolated and characterized. Isolates T4, T15 and M5 showed extracellular antimicrobial activity against the fish pathogen Carnobacterium maltaromaticum CECT 4020, by the agar well diffusion assay. They were identified as Gram‐positive catalase‐negative bacilli belonging to the genus Carnobacterium (based on 99.93% identity with the 16S rRNA gene sequence of C. maltaromaticum DSM 20342T). After 48 hr of incubation in MRS broth, the highest antimicrobial activity titre (327,680 AU/ml) was recorded for T4, followed by T15 and M5. The antimicrobial agents produced by these isolates were thermostable peptides. LC‐MS/MS peptide analysis indicated that T4 and T5 bacteriocins belonged to the class IIa. The identified peptides for T4 bacteriocin included almost the entire divercin V41 sequence, which was previously believed to be exclusively produced by Carnobacterium divergens. In addition, no negative fish health effects were observed after administrating T4 to zebrafish. Among the isolates from healthy Patagonian trout, due to its early bacteriocin production, its greater inhibition titre and its innocuous feature, Carnobacterium sp. T4 emerged as the most promising isolate for in vivo challenges targeting its further aquaculture application.

中文翻译:

产生细菌素的食肉杆菌(Carnobacterium spp)的抗菌活性。分离自健康的巴塔哥尼亚鳟鱼及其在水产养殖中的应用潜力

在这项研究中,分离并鉴定了巴塔哥尼亚鳟鱼肠中显示的鱼病原体抗菌活性的细菌。通过琼脂孔扩散分析,分离物T4,T15和M5对鱼类病原体麦芽线菌CECT 4020表现出细胞外抗菌活性。它们被鉴定为属于食肉杆菌属的革兰氏阳性过氧化氢酶阴性杆菌(基于与麦芽糖梭菌DSM 20342 T的16S rRNA基因序列的99.93%相同性)。在MRS肉汤中孵育48小时后,记录到的T4的最高抗菌活性滴度(327,680 AU / ml),然后是T15和M5。这些分离物产生的抗微生物剂是热稳定肽。LC-MS / MS肽分析表明T4和T5细菌素属于IIa类。T4细菌素的已鉴定肽几乎包括整个曲霉素V41序列,以前认为这是由分枝杆菌(Carnobacterium divergens)专门产生的。此外,在斑马鱼上施用T4后,未观察到对鱼类健康的负面影响。在健康的巴塔哥尼亚鳟鱼的分离物中,由于其早期细菌素的产生,更大的抑制效价和无毒特征,即食肉杆菌sp。T4成为针对其进一步水产养殖应用的体内挑战的最有希望的分离物。
更新日期:2020-10-11
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