Viral Vectors for Neural Circuit Mapping and Recent Advances in Trans-synaptic Anterograde Tracers.,Neuron

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Viral Vectors for Neural Circuit Mapping and Recent Advances in Trans-synaptic Anterograde Tracers.
Neuron ( IF 16.2 ) Pub Date : 2020-08-04 , DOI: 10.1016/j.neuron.2020.07.010
Xiangmin Xu ₁ , Todd C Holmes ₂ , Min-Hua Luo ₃ , Kevin T Beier ₂ , Gregory D Horwitz ₄ , Fei Zhao ₅ , Wenbo Zeng ₆ , May Hui ₇ , Bert L Semler ₈ , Rozanne M Sandri-Goldin ₈

Affiliation

Department of Anatomy and Neurobiology, School of Medicine, University of California, Irvine, Irvine, CA 92697-1275, USA; Department of Microbiology and Molecular Genetics, School of Medicine, University of California, Irvine, Irvine, CA 92697-4025, USA; Department of Biomedical Engineering, University of California, Irvine, Irvine, CA 92697-2715, USA; The Center for Neural Circuit Mapping, University of California, Irvine, Irvine, CA 92697, USA.
Department of Physiology and Biophysics, University of California, Irvine, Irvine, CA 92697-4560, USA; The Center for Neural Circuit Mapping, University of California, Irvine, Irvine, CA 92697, USA.
State Key Laboratory of Virology, Wuhan Institute of Virology, CAS Center for Excellence in Brain Science, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan 430071, China; The Center for Neural Circuit Mapping, University of California, Irvine, Irvine, CA 92697, USA.
The Washington National Primate Research Center, University of Washington, Seattle, WA 98195, USA; Department of Physiology & Biophysics, University of Washington, Seattle, WA 98195, USA; The Center for Neural Circuit Mapping, University of California, Irvine, Irvine, CA 92697, USA.
School of Basic Medical Sciences, Capital Medical University, Beijing 102206, China; Chinese Institute for Brain Research (CIBR), Beijing 102206, China.
State Key Laboratory of Virology, Wuhan Institute of Virology, CAS Center for Excellence in Brain Science, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan 430071, China.
Department of Physiology and Biophysics, University of California, Irvine, Irvine, CA 92697-4560, USA.
Department of Microbiology and Molecular Genetics, School of Medicine, University of California, Irvine, Irvine, CA 92697-4025, USA; The Center for Neural Circuit Mapping, University of California, Irvine, Irvine, CA 92697, USA.

Viral tracers are important tools for neuroanatomical mapping and genetic payload delivery. Genetically modified viruses allow for cell-type-specific targeting and overcome many limitations of non-viral tracers. Here, we summarize the viruses that have been developed for neural circuit mapping, and we provide a primer on currently applied anterograde and retrograde viral tracers with practical guidance on experimental uses. We also discuss and highlight key technical and conceptual considerations for developing new safer and more effective anterograde trans-synaptic viral vectors for neural circuit analysis in multiple species.

中文翻译：

神经回路映射的病毒载体和跨突触顺行示踪剂的最新进展。

病毒示踪剂是神经解剖图和基因有效载荷传递的重要工具。转基因病毒允许细胞类型特异性靶向并克服非病毒示踪剂的许多限制。在这里，我们总结了为神经回路映射开发的病毒，我们提供了当前应用的顺行和逆行病毒示踪剂的入门指南，并提供了实验用途的实际指导。我们还讨论并强调了开发新的更安全、更有效的顺行跨突触病毒载体用于多个物种的神经回路分析的关键技术和概念考虑因素。

更新日期：2020-09-23

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