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Electrochemiluminescence immunoassay of human chorionic gonadotropin using silver carbon quantum dots and functionalized polymer nanospheres
Microchimica Acta ( IF 5.7 ) Pub Date : 2020-08-01 , DOI: 10.1007/s00604-020-04450-0
Dongmiao Qin 1 , Xiaohua Jiang 1 , Guichun Mo 1 , Xiangfei Zheng 1 , Biyang Deng 1
Affiliation  

A composite, reduced graphene oxide (rGO) doped with silver nanoparticles (Ag NPs), was prepared by using binary reductants of sodium citrate and hydrazine hydrate. Carbon quantum dots (CQDs) synthesized by papaya peel combined with silver ions to form a CQDs-loaded silver nanoparticle (AgCQDs) nanocomposite. Polymer nanospheres (PNS) were generated via the infinite coordination polymer of ferrocene dicarboxylic acid and employed as carriers to load AgCQDs. The prepared AgCQDs@PNS–PEI has good biocompatibility and electrical conductivity and can be used as a matrix for the immobilization of a secondary antibody (Ab2). A sandwich-type electrochemiluminescence (ECL) immunosensor using AgCQDs@PNS–PEI nanocomposite as probe has been developed for the detection of human chorionic gonadotropin (HCG). The proposed immunosensor exhibits a linear range from 0.00100 to 500 mIU mL−1 and the detection limit is 0.33 μIU mL−1 (S/N = 3) under optimal conditions. The sensor exhibits excellent selectivity, good reproducibility, and high stability. These features demonstrate that the proposed method has promising potential for clinical protein detection and displays a new strategy to fabricate an immunosensor. Graphical abstract Graphical abstract

中文翻译:

使用银碳量子点和功能化聚合物纳米球对人绒毛膜促性腺激素进行电化学发光免疫分析

通过使用柠檬酸钠和水合肼的二元还原剂制备了掺杂有银纳米颗粒 (Ag NPs) 的复合还原氧化石墨烯 (rGO)。由木瓜皮合成的碳量子点 (CQDs) 与银离子结合形成负载 CQDs 的银纳米粒子 (AgCQDs) 纳米复合材料。聚合物纳米球 (PNS) 是通过二茂铁二羧酸的无限配位聚合物生成的,并用作负载 AgCQD 的载体。制备的 AgCQDs@PNS-PEI 具有良好的生物相容性和导电性,可用作固定二抗 (Ab2) 的基质。已经开发了一种使用 AgCQDs@PNS-PEI 纳米复合材料作为探针的夹心型电化学发光 (ECL) 免疫传感器,用于检测人绒毛膜促性腺激素 (HCG)。所提出的免疫传感器的线性范围为 0.00100 至 500 mIU mL-1,在最佳条件下检测限为 0.33 μIU mL-1 (S/N = 3)。该传感器具有优异的选择性、良好的重现性和高稳定性。这些特征表明所提出的方法在临床蛋白质检测方面具有广阔的潜力,并展示了一种制造免疫传感器的新策略。图形摘要图形摘要
更新日期:2020-08-01
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