As biological synthesis has become an alternative to chemical and physical methods for synthesizing nanoparticles, this work describes the synthesis of Au/CuO/ZnO nanoparticles using Verbena officinalis extract. The synthesized Au/CuO/ZnO nanoparticles were characterized using Ultraviolet–Visible, Fourier Transform-Infrared, Transmission Electron Microscopy and Atomic Force Microscopy. The influence of Au/CuO/ZnO nanoparticles on cell viability was evaluated in vitro, using the established cell line – Jurkat (ATCC® TIB-152™). The Annexin V binding test confirmed the previous results of the MTT assay, which indicate that the studied complex of Au/CuO/ZnO nanoparticles has a strong cytotoxic effect on the Jurkat cell line. The type of death and the effectiveness of cell elimination depended both on the concentration of the complex and the duration of culture.

由于生物合成已成为合成纳米粒子的化学和物理方法的替代方法，因此这项工作描述了使用马鞭草合成Au / CuO / ZnO纳米粒子的方法。提取。合成的Au / CuO / ZnO纳米粒子使用紫外-可见，傅里叶变换红外，透射电子显微镜和原子力显微镜进行表征。使用已建立的细胞系Jurkat（ATCC®TIB-152™），在体外评估了Au / CuO / ZnO纳米颗粒对细胞活力的影响。膜联蛋白V结合试验证实了MTT测定的先前结果，这表明所研究的Au / CuO / ZnO纳米颗粒复合物对Jurkat细胞系具有强烈的细胞毒性作用。死亡的类型和细胞消除的有效性取决于复合物的浓度和培养时间。