Microalgae are photosynthetic microorganisms known for their variety of compounds that can be useful for food, feed, pharmaceuticals, and fuel industries. Microalgae production costs have been one of the major obstacles to large-scale commercial production. Therefore, several studies are still being performed aiming to increase biomass by developing novel photobioreactor design and microalgae cultivation techniques. This work’s purpose is to increase Neochloris oleoabundans biomass. Different nutrient-feeding regimes were tested in N. oleoabundans cultivation in Erlenmeyer flasks and bench-scale tubular photobioreactor. In Erlenmeyer flasks, the best concentrations of nitrate and phosphate were 8.82 mM and 5.16 mM, respectively. In bench-scale tubular photobioreactor, NaNO3 proved to be the best nitrogen source, in comparison with (NH4)2SO4 and NH4NO3. Still in the photobioreator, the addition of nitrate by fed-batch process combined with automated system of CO2 feeding showed to be of utmost importance for insuring a high density of N. oleoabundans. The essential nutrients evaluated in this work play an important role on N. oleoabundans biomass accumulation, as well as the photobioreactor configuration and feeding regimes.

中文翻译：

---

不同氮:磷:碳饲养策略下的新绿藻生长评价

微藻是光合微生物，以其可用于食品、饲料、制药和燃料工业的各种化合物而闻名。微藻生产成本一直是大规模商业化生产的主要障碍之一。因此，仍在进行一些旨在通过开发新型光生物反应器设计和微藻培养技术来增加生物量的研究。这项工作的目的是增加 Neochloris oleoabundans 的生物量。在 Erlenmeyer 烧瓶和台式管式光生物反应器中的 N. oleoabundans 培养中测试了不同的营养供给方案。在锥形瓶中，硝酸盐和磷酸盐的最佳浓度分别为 8.82 mM 和 5.16 mM。在实验室规模的管式光生物反应器中，NaNO3 被证明是最好的氮源，与 (NH4)2SO4 和 NH4NO3 相比。仍然在光生物反应器中，通过分批补料工艺添加硝酸盐与 CO2 自动补料系统相结合，表明对于确保高密度 N.oleoabundans 至关重要。在这项工作中评估的必需营养素对 N. oleoabundans 生物量积累以及光生物反应器配置和饲养制度起着重要作用。