Myocardial infarction is a clinical form of necrosis in the myocardium caused by an imbalance between the coronary blood supply and myocardial demand. Garcinol is a polyisoprenylated benzophenone found in the fruit of Garcinia indica, which is abundant in tropical regions. This fruit contains high levels of garcinol, isoxanthochymol, isogarcinol, hydroxycitric acid and xanthochymol. Garcinol and hydroxycitric acid have been shown to have antioxidant effects. In this study, rats were assigned to sham, control, low-dose, high-dose and positive control groups. Hemodynamic and apoptotic markers were evaluated, and histopathological analysis was conducted. The mRNA and protein levels of caspase-3, Bax, Bcl-2 and cleaved caspase-3 were quantified. Garcinol treatment increased the heart rate and improved the maximum rate of increase in left-ventricle (LV) pressure (+dp/dt_max), maximum rate of decrease in LV pressure (–dp/dt_max), LV ejection fraction and LV systolic pressure in rats with induced heart failure. Garcinol treatment reversed body, liver and heart weight changes, resulting in returns to near-normal levels. In the garcinol treatment group, the number of broken fibers, extent of inflammatory cell infiltration and rate of apoptosis remained within normal ranges. Garcinol reduced the cross-sectional areas of cardiomyocytes, and reduced interstitial fibrosis to a normal level. The mRNA and protein levels of cleaved caspase-3, caspase-3 and Bax were reduced, whereas those of Bcl-2 were increased, following high-dose (100 mg/kg) garcinol treatment. These findings suggest that garcinol effectively prevents apoptosis in rats with isoproterenol-induced heart failure and in cardiac H9C2 cells.

中文翻译：

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大蒜素对心肌梗死后异丙肾上腺素所致心力衰竭大鼠的心脏保护作用。

心肌梗塞是由冠状动脉供血和心肌需求之间的不平衡引起的心肌坏死的临床形式。藤黄酚是一种在藤黄果中发现的聚异戊二烯基二苯甲酮。 ，在热带地区丰富。这种水果中含有大量的藤黄酚，异黄腐酚，异黄霉素，羟基柠檬酸和黄腐酚。大蒜油和羟基柠檬酸已被证明具有抗氧化作用。在这项研究中，将大鼠分为假，对照组，低剂量，高剂量和阳性对照组。评估血流动力学和凋亡标志物，并进行组织病理学分析。定量caspase-3，Bax，Bcl-2和裂解的caspase-3的mRNA和蛋白水平。大蒜素治疗可提高心率，并改善左心室（LV）压力的最大增加速率（+ d p / d t _max），最大左心室压力的减小速率（–d p / d t _max）），诱发心力衰竭的大鼠左室射血分数和左室收缩压。大蒜素治疗逆转了身体，肝脏和心脏的重量变化，导致恢复到接近正常水平。在大蒜素治疗组中，纤维断裂的数量，炎性细胞浸润的程度和凋亡率均保持在正常范围内。大蒜素可减少心肌细胞的横截面积，并将间质纤维化减少至正常水平。大剂量（100 mg / kg）大蒜素处理后，裂解的caspase-3，caspase-3和Bax的mRNA和蛋白水平降低，而Bcl-2的mRNA和蛋白水平升高。这些发现表明，在由异丙肾上腺素引起的心力衰竭的大鼠和心脏H9C2细胞中，藤黄酚可有效防止细胞凋亡。