The present study was designed to explore the role of microRNA-197-3p in regulating the epithelial–mesenchymal cellular transition in ovarian cancer. The results showed that miR-197 to be significantly (P < 0.05) downregulated in human ovarian cancer tissues and cell lines. Overexpression of miR-197 significantly (P < 0.05) reduced the proliferation of OVACAR-3 cancer cells. Additionally, the colony formation of the OVACAR-3 cells was inhibited by 59% relative to control. The migration and invasion of the OVACAR-3 cells was inhibited by 64% and 72%, respectively, upon miR-197 overexpression. Western blot analysis showed miR-197 was found to upregulate the expression of E-cadherin, while the expression of N-cadherin, vimentin, and snail proteins was found to decrease significantly (P < 0.05). TargetScan analysis together with dual luciferase assay revealed that miR-197 exerts its effects by targeting ABCA7 in ovarian cancer. ABCA7 was significantly (P < 0.05) overexpressed in ovarian cancer tissues and cell lines. However, silencing of ABCA7 resulted in significant inhibition of cell proliferation, migration, and invasion. Nonetheless, overexpression of ABCA7 could abolish the tumor-suppressive effects of miR-197 on the OVACAR-3 cells. Taken together, miR-197 acts a tumor-suppressive in ovarian cancer and points towards its therapeutic implications in the treatment of ovarian cancer.

本研究旨在探讨 microRNA-197-3p 在调节卵巢癌上皮-间充质细胞转化中的作用。结果表明，miR-197 在人卵巢癌组织和细胞系中显着下调（P < 0.05）。miR-197的过表达显着（P  < 0.05）降低了OVACAR-3癌细胞的增殖。此外，与对照相比，OVACAR-3 细胞的集落形成被抑制了 59%。miR-197 过表达后，OVACAR-3 细胞的迁移和侵袭分别被抑制了 64% 和 72%。蛋白质印迹分析显示 miR-197 上调 E-cadherin 的表达，而 N-cadherin、vimentin 和 snail 蛋白的表达显着降低。P  < 0.05)。TargetScan 分析和双荧光素酶分析显示 miR-197 通过靶向 ABCA7 在卵巢癌中发挥作用。ABCA7 在卵巢癌组织和细胞系中显着（P < 0.05）过表达。然而，ABCA7 的沉默导致细胞增殖、迁移和侵袭的显着抑制。尽管如此，ABCA7 的过表达可以消除 miR-197 对 OVACAR-3 细胞的肿瘤抑制作用。总之，miR-197 在卵巢癌中起到肿瘤抑制作用，并指出其在卵巢癌治疗中的治疗意义。