To detect the presence of antibodies in blood against SARS-CoV-2 in a highly sensitive and specific manner, here we describe a robust, inexpensive ($200), 3D-printable portable imaging platform (TinyArray imager) that can be deployed immediately in areas with minimal infrastructure to read coronavirus antigen microarrays (CoVAMs) that contain a panel of antigens from SARS-CoV-2, SARS-1, MERS, and other respiratory viruses. Application includes basic laboratories and makeshift field clinics where a few drops of blood from a finger prick could be rapidly tested in parallel for the presence of antibodies to SARS-CoV-2 with a test turnaround time of only 2–4 h. To evaluate our imaging device, we probed and imaged coronavirus microarrays with COVID-19-positive and negative sera and achieved a performance on par with a commercial microarray reader 100× more expensive than our imaging device. This work will enable large scale serosurveillance, which can play an important role in the months and years to come to implement efficient containment and mitigation measures, as well as help develop therapeutics and vaccines to treat and prevent the spread of COVID-19.

中文翻译：

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用于高度特异性 COVID-19 抗体测试的模块化微阵列成像系统。

为了以高度灵敏和特异的方式检测血液中是否存在针对 SARS-CoV-2 的抗体，我们在这里描述了一种强大、廉价（200 美元）、可 3D 打印的便携式成像平台（TinyArray 成像仪），可以立即部署在一些区域具有最少的基础设施来读取冠状病毒抗原微阵列（CoVAM），其中包含一组来自 SARS-CoV-2、SARS-1、MERS 和其他呼吸道病毒的抗原。应用包括基础实验室和临时现场诊所，可以同时快速测试刺破手指的几滴血液是否存在 SARS-CoV-2 抗体，测试周转时间仅为 2-4 小时。为了评估我们的成像设备，我们使用 COVID-19 阳性和阴性血清对冠状病毒微阵列进行探测和成像，其性能与比我们的成像设备贵 100 倍的商用微阵列读取器相当。这项工作将实现大规模的血清监测，这可以在未来数月和数年中发挥重要作用，以实施有效的遏制和缓解措施，并帮助开发治疗和预防 COVID-19 传播的疗法和疫苗。