The development of Cas9/gRNA-mediated gene-drive systems has bolstered the advancement of genetic technologies for controlling vector-borne pathogen transmission. These include population suppression approaches, genetic analogs of insecticidal techniques that reduce the number of vector insects, and population modification (replacement/alteration) approaches, which interfere with competence to transmit pathogens. We developed a recoded gene-drive rescue system for population modification in the malaria vector, Anopheles stephensi, that relieves the load in females caused by integration of the drive into the kynurenine hydroxylase gene by rescuing its function. Non-functional resistant alleles are eliminated via a dominantly-acting maternal effect combined with slower-acting standard negative selection, and a functional resistant allele does not prevent drive invasion. Small cage trials show that single releases of gene-drive males robustly result in efficient population modification with >95% of mosquitoes carrying the drive within 5-11 generations over a range of initial release ratios.

中文翻译：

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疟疾蚊按蚊中有效的种群修饰基因驱动拯救系统

Cas9 / gRNA介导的基因驱动系统的发展促进了控制载体传播的病原体传播的遗传技术的发展。这些措施包括种群抑制方法，减少媒介昆虫数量的杀虫技术的遗传类似物，以及干扰传播病原体能力的种群改良（替代/替代）方法。我们开发了用于疟疾媒介Anopheles stephensi中的种群修饰的重新编码的基因驱动抢救系统，该系统通过挽救其功能而减轻了由于驱动器整合到犬尿氨酸羟化酶基因中而导致的女性负担。非功能性抗性等位基因通过显性作用的母体效应和作用较慢的标准阴性选择而消除，抗性等位基因不能阻止驱动器入侵。小笼试验表明，单次释放基因驱动雄性有力地导致了种群的有效改变，在初始释放率范围内，> 95％的蚊子在5到11代内携带了该驱动力。