Abstract

Achyrocline flaccida (Weinm) DC. essential oil was obtained by hydrodistillation of wild flowers from southern Brazil. We explored, for the first time, the phytochemical composition, toxicity, resistance to oxidative stress in Caenorhabditis elegans, and antimycobacterial activities of A. flaccida essential oil. Twenty-four compounds were identified by GC-FID-MS and the major constituents identified were α-pinene (41.10%) and caryophyllene (30.52%). The essential oil showed no signs of genotoxicity in the comet assay and presented relevant antioxidant capacity since it prevented ROS production in the C. elegans model. Furthermore, the minimal inhibitory concentrations (MIC) results showed that M. abscessus, M. massiliense, and M. fortuitum had their growth inhibited by A. flaccida essential oil. Therefore, the essential oil of this plant is a promising alternative in the search for new compounds capable of decreasing oxidative stress and treating mycobacteriosis.

摘要

Achyrocline flaccida (Weinm) DC。精油是通过水蒸馏来自巴西南部的野花获得的。我们探讨，对于第一次，植物化学物质的组合物，毒性，氧化胁迫抗性在秀丽隐杆线虫，和抗分支杆菌活性A.松弛部精油。GC-FID-MS 鉴定了 24 种化合物，鉴定的主要成分为 α-蒎烯 (41.10%) 和石竹烯 (30.52%)。精油在彗星试验中没有表现出遗传毒性的迹象，并表现出相关的抗氧化能力，因为它阻止了秀丽隐杆线虫模型中的 ROS 产生。此外，最低抑菌浓度 (MIC) 结果表明，脓肿分枝杆菌、M. massiliense和M. fortuitum的生长受到A. flaccida精油的抑制。因此，这种植物的精油是寻找能够减少氧化应激和治疗分枝杆菌病的新化合物的有前途的替代品。