Abstract

The study was intended to find in vitro antioxidant, cytotoxicity and safety profile of Polystichum braunii roots extracts and chemically characterise it by GC–MS and FTIR spectroscopy. Acute and sub-chronic (90-day oral repeated dose at 150, 300 and 600 mg/kg) toxicity studies of P. braunii methanol extract (PBME) were performed in Wistar rats by adopting OECD guidelines. The PBME exerted the highest in vitro antioxidant potential. There was no substantial change in blood parameters and body weight except a notable (p < 0.01) rise in ALT, ALP, MDA and HDL, and decrease in cholesterol and LDL (p < 0.05) in animals sub-chronically treated with PBME at 600 mg/kg dose. The IC₅₀ in HepG2 was more than 1000 µg/ml. The GC–MS analysis revealed Pentadecanoic acid and 9,12-Octadecadienoic acid in PBME. The P. braunii might be safe in acute usage, however, monitoring of liver function tests should be carried out during long-term use.

摘要

该研究的目的是寻找布氏多刺提取物的体外抗氧化性、细胞毒性和安全性，并通过 GC-MS 和 FTIR 光谱对其进行化学表征。急性和亚慢性毒性研究（90天口服重复在150，300和600mg / kg剂量）P.藻甲醇提取物（PBME）通过采用OECD指南在Wistar大鼠中进行的。PBME 发挥了最高的体外抗氧化潜力。血液参数和体重没有实质性变化，除了 在 600 年用 PBME 亚慢性治疗的动物 中 ALT、ALP、MDA 和 HDL显着升高( p < 0.01)，以及胆固醇和低密度脂蛋白降低 ( p < 0.05)毫克/公斤剂量。集成电路HepG2 中的₅₀超过 1000 µg/ml。GC-MS 分析显示 PBME 中含有十五烷酸和 9,12-十八碳二烯酸。该P.藻可能在急性使用是安全的，但如果监测肝功能检查应长期使用过程中进行。