Abstract

In this study, we evaluated the pathogenicity of 27 isolates of Fusarium incarnatum-equiseti Species Complex (FIESC) against N. corniger and S. frugiperda: four Fusarium sulawesiense (= FIESC 16), six Fusarium pernambucanum (= FIESC 17) and seventeen Fusarium caatingaense (= FIESC 20). Suspensions of 1 × 10⁷ conidia/mL⁻¹ were used for the pathogenicity tests. Cumulative mortality data on the fifth day were used for analysis of variance (ANOVA) and the means were compared by the Scott–Knott test (p ≤ .05). The variables (isolate and time) were submitted to regression analysis. The results revealed the absence of pathogenicity for isolates against S. frugiperda. On the other hand, all isolates tested were pathogenic to N. corniger with cumulative mortality confirmed at 38.22 − 96.00%. The isolates that caused the highest mortality values of N. corniger on the fifth day of evaluation also killed the insects faster. The highest confirmed cumulative mortality values were observed for URM 7560 from F. sulawesiense (88.89%), URM 7556 from F. pernambucanum (96.00%) and URM 6783 from F. caatingaense (84.89%). The effectiveness of these isolates will be compared in future field tests.

摘要

在这项研究中，我们评估了 27 株板栗镰刀菌(FIESC) 对N.corniger和S. frugiperda的致病性：4株苏拉威辛镰刀菌(= FIESC 16)、6株高山镰刀菌(= FIESC 17) 和 17株镰刀菌caatingaense (= FIESC 20)。1×10 ⁷分生孢子/mL ^-1的悬浮液用于致病性测试。第五天的累积死亡率数据用于方差分析（ANOVA），平均值通过 Scott-Knott 检验（p ≤ .05)。将变量（隔离和时间）提交给回归分析。结果表明，对S. frugiperda的分离株没有致病性。另一方面，所有测试的分离株都对N.corniger具有致病性，累积死亡率确认为 38.22 - 96.00%。在评估的第五天导致N.corniger死亡率最高的分离株也更快地杀死了昆虫。F. sulawesiense的 URM 7560 (88.89%)、F. pernambucanum 的 URM 7556 ( 96.00%) 和F. caatingaense的 URM 6783 (84.89%) 的累积死亡率最高。这些分离物的有效性将在未来的现场测试中进行比较。