Fish offal and other high protein substrates are generally not suitable for anaerobic digestion because of the high levels of ammonia produced as a result of their biodegradation. In order to efficiently use these types of substrates to produce methane, co-digestion is used to balance the amounts of carbon and nitrogen in the feedstock. In this experiment an optimization procedure for maximizing the methane potential of fish offal, using river tamarind as the co-substrates was developed. Our experimental design tested the effects of substrate to substrate mixtures, as well as overall substrate to inoculum combinations, on the methane potentials. This was performed using batch style biochemical methane potential assays, which employed a methodology developed in our laboratory. The optimum of the 25 combinations tested was 50% fish offal to 50% river tamarind at a substrate to inoculum ratio of 0.03, with a specific methane yield of 144 ± 6 NmL/gFM (330 ± 14 NmL/goDM). This gave much improvement when compared with the fish offal alone, which reached 63 ± 4 NmL/gFM (317 ± 20 NmL/goDM) at maximum. These results indicate that with the correct mixture, rivertamarind is a suitable co-substrate for anaerobic co-digestion of fish offal.

中文翻译：

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通过优化罗望子河（Leucaena leucocephala）和海豚鱼（Coryphaena hippurus）内脏的混合比，通过厌氧消化来生产生物甲烷

鱼内脏和其他高蛋白底物通常不适合厌氧消化，因为它们的生物降解会产生大量氨。为了有效地使用这些类型的底物来生产甲烷，共消化用于平衡原料中碳和氮的量。在该实验中，开发了一种优化程序，该程序使用河罗望子作为共底物来最大化鱼内脏的甲烷潜力。我们的实验设计测试了底物与底物混合物以及整个底物与接种物组合对甲烷电势的影响。这是使用批量式生化甲烷潜力测定法进行的，该测定法采用了我们实验室开发的方法。测试的25种组合的最佳条件是底物与接种物的比例为0.03时，鱼内脏为50％，河罗望子为50％，甲烷的比甲烷产量为144±6 NmL / gFM（330±14 NmL / goDM）。与单独的鱼内脏相比最大改进为63±4 NmL / gFM（317±20 NmL / goDM）。这些结果表明，使用正确的混合物，河罗望子素是鱼内脏厌氧共消化的合适共底物。