Two novel strains AV382 and AV436 were isolated from a submerged industrial bioreactor for production of apple cider vinegar in Kopivnik (Slovenia). Both strains showed very high (≥98.2%) 16S rRNA gene sequence similarities with Komagataeibacter species, but lower 16S–23S rRNA gene internal transcribed spacer (ITS). The highest similarity of the 16S–23S rRNA gene ITS of AV382 was to Komagataeibacter kakiaceti LMG 26206^T (91.6%), of AV436 to Komagataeibacter xylinus LMG 1515^T (93.9%). The analysis of genome sequences confirmed that AV382 is the most closely related to K. kakiaceti (ANIb 88.2%) and AV436 to K. xylinus (ANIb 91.6%). Genome to genome distance calculations exhibit for both strains ≤47.3% similarity to all type strains of the genus Komagataeibacter. The strain AV382 can be differentiated from its closest relatives K. kakiaceti and Komagataeibacter saccharivorans by its ability to form 2-keto and 5-keto-D-gluconic acids from glucose, incapability to grow in the presence of 30% glucose, formation of C_19:0 cyclo ω8c fatty acid and tolerance of up to 5% acetic acid in the presence of ethanol. The strain AV436 can be differentiated from its closest relatives K. xylinus, Komagataeibacter sucrofermentans, and Komagataeibacter nataicola by its ability to form 5-keto-D-gluconic acid, growth on 1-propanol, efficient synthesis of cellulose, and tolerance to up to 5% acetic acid in the presence ethanol. The major fatty acid of both strains is C_18:1ω7c. Based on a combination of phenotypic, chemotaxonomic and phylogenetic features, the strains AV382^T and AV436^T represent novel species of the genus Komagataeibacter, for which the names Komagataeibactermelaceti sp. nov. and Komagataeibacter melomenusus are proposed, respectively. The type strain of Komagataeibacter melaceti is AV382^T (= ZIM B1054^T = LMG 31303^T = CCM 8958^T) and of Komagataeibacter melomenusus AV436^T (= ZIM B1056^T = LMG 31304^T = CCM 8959^T).

中文翻译：

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melaceti sp。的描述 十一月 和komagataeibacter melomenusus sp。十一月 与苹果酒醋隔离。

从科比夫尼克（斯洛文尼亚）的水下工业生物反应器中分离出两种新型菌株AV382和AV436，用于生产苹果醋。两种菌株显示出非常高（≥98.2％）与16S rRNA基因的序列相似Komagataeibacter物种，但较低的16S-23S rRNA基因内转录间隔区（ITS）。AV382的16S–23S rRNA基因ITS的最高相似度为拟杆菌Komgataeibacter kakiaceti LMG 26206 ^T（91.6％），AV436的16S–23S rRNA基因ITS最高相似度为Komagataeibacter xylinus LMG 1515 ^T（93.9％）。基因组序列分析证实AV382与K. kakiaceti（ANIb 88.2％）和AV436与K. xylinus关系最密切（ANIb 91.6％）。基因组以基因组距离计算表现出对两种菌株≤47.3％相似属的所有型菌株Komagataeibacter。可以将AV382菌株与其近亲K. kakiaceti和Komagataeibacter saccharivorans区别开来，因为它能够从葡萄糖中形成2-酮和5-酮-D-葡萄糖酸，在30％的葡萄糖存在下不能生长，不能形成C。_19：0环ω 8C脂肪酸和至多5％乙酸在乙醇的存在下的耐受性。AV436菌株可以与其近亲K. xylinus，Komagataeibacter sucrofermentans和Komagataeibacter nataicola区分。具有形成5-酮-D-葡萄糖酸的能力，在1-丙醇上的生长，纤维素的高效合成以及在乙醇存在下对高达5％乙酸的耐受性。两种菌株的主要脂肪酸是C _18：1和ω 7C。基于表型，化学分类和系统发育特征的组合，菌株AV382 ^Ť和AV436 ^Ť代表属的新种Komagataeibacter，为此，名称Komagataeibacter melaceti藻。十一月 分别提出了Memomenus和Komagataeibacter melomenusus。melaceti的Komagataeibacter型细菌是AV382 ^T（= ZIM B1054 ^T = LMG 31303^T = CCM 8958 ^T）和Komagataeibacter melomenusus AV436 ^T（= ZIM B1056 ^T = LMG 31304 ^T = CCM 8959 ^T）。