Auricularia auricula (A. auricula) has been reported to have positive health effects. Therefore, this study was conducted to explore possible mechanisms of A. auricula‐induced anticancer activity in hepatocellular carcinoma (HCC) cells. First, using proliferative assay including MTT assay and real‐time cell electronic sensing technique, we founded that A. auricula has an antiproliferative effect on various cancer cell lines. Among five cancer cell lines, we focused on huh‐7 cell line, HCC cell line, due to that A. auricula has most dramatic antiproliferative effects on huh‐7 cell line. Following experiments, we founded that its antiproliferative effects was related with apoptosis‐inducing activities. For more investigation, a two‐dimensional electrophoresis based‐proteomic analysis (2DE‐GE) was employed for identification of possible target‐related proteins of A. auricula‐induced apoptosis. Among seven identified proteins, we focused on peroxiredoxin1 (PRDX1), which has been known as an anti‐oxidative enzyme. We confirmed downregulation of expression of PRDX1 following A. auricula treatment in mRNA and protein level. In order to obtain a more validation of the correlation of A. auricula‐induced cell death and anti‐oxidative enzyme. We investigated the level of anti‐oxidative enzymes, total glutathione (GSSG/GSH), and superoxide dismutase (SOD) levels in treated cells and PRDX1 gene‐silenced cells. GSH and SOD levels were decreased in the treated cells and PRDX1 gene‐silenced cells. Our findings suggest that A. auricula is a potent inducer of apoptosis in HCC cells via PRDX1‐inhibition pathways.

中文翻译：

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Auricularia auricula通过调节peroxiredoxin1来增加人肝癌细胞的凋亡。

据报道，耳廓木耳（A. auricula）具有积极的健康作用。因此，本研究旨在探讨金黄色葡萄球菌诱导的肝细胞癌（HCC）细胞抗癌活性的可能机制。首先，我们使用包括MTT分析和实时细胞电子传感技术在内的增殖分析方法，发现金耳曲霉对多种癌细胞具有抗增殖作用。在这五种癌细胞系中，由于该A. auricula，我们专注于huh-7细胞系，HCC细胞系对huh-7细胞系具有最显着的抗增殖作用。经过实验，我们发现其抗增殖作用与细胞凋亡诱导活性有关。为了进行更多的研究，我们使用了基于二维电泳的蛋白质组学分析（2DE-GE）来鉴定金黄色葡萄球菌诱导的细胞凋亡的靶标相关蛋白。在鉴定出的七个蛋白质中，我们重点研究了过氧化物酶1（PRDX1），它被称为抗氧化酶。我们下面证实PRDX1的表达下调黑木耳中的mRNA处理和蛋白质水平。为了获得对金耳草相关性的更多验证诱导的细胞死亡和抗氧化酶。我们研究了处理过的细胞和PRDX1基因沉默的细胞中的抗氧化酶，总谷胱甘肽（GSSG / GSH）和超氧化物歧化酶（SOD）的水平。在处理过的细胞和PRDX1基因沉默的细胞中，GSH和SOD水平降低。我们的发现表明，金耳曲霉通过PRDX1抑制途径是HCC细胞凋亡的有效诱导剂。