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All-in-one microfluidic nucleic acid diagnosis system for multiplex detection of sexually transmitted pathogens directly from genitourinary secretions
Talanta ( IF 6.1 ) Pub Date : 2020-08-03 , DOI: 10.1016/j.talanta.2020.121462
Xin Ye , Yang Li , Lijuan Wang , Xueen Fang , Jilie Kong

Sexually transmitted infections are a serious public health concern worldwide, especially in young people. More than 30 pathogens can cause sexually transmitted diseases and co-infection often occurs. Therefore, the development of fast, low-cost and easy-to-use diagnostic screening methods is urgently needed for disease prevention and control. Herein, we established an all-in-one microfluidic nucleic acid diagnosis system, which could simultaneously detect Chlamydia trachomatis, Neisseria gonorrhoeae, Mycoplasma hominis and Ureaplasma urealyticum directly from genitourinary secretions with minimal manual manipulations. This system integrated nucleic acid extraction, amplification, and detection on a single microfluidic chip and could be automatically performed in an integrated detection device. This novel diagnosis tool showed good detection limits, stability (coefficient of variation <6%), specificity (no cross-reaction with 23 other pathogens for each target) and resistance to interference by other substances and the diagnostic efficacy was similar to that of PCR. The turn-around time was reduced to 50 min from sample to answer with automated testing steps. This novel diagnosis tool has the advantages of highly integrated, automated, sample-to-answer detection, and could thus replace the traditional method. This could significantly improve the prevention and control of sexually transmitted diseases.



中文翻译:

多合一微流核酸诊断系统,可直接从泌尿生殖道分泌物中多重检测性传播病原体

性传播感染是世界范围内严重的公共卫生问题,尤其是在年轻人中。超过30种病原体可以导致性传播疾病,并且经常发生合并感染。因此,迫切需要开发快速,低成本和易于使用的诊断筛选方法来预防和控制疾病。在此,我们建立了所有功能于一个微流体核酸诊断系统,它可以同时检测沙眼衣原体淋病奈瑟氏菌人型支原体解脲支原体直接从生殖泌尿生殖道分泌物进行,几乎不需要人工操作。该系统在单个微流控芯片上集成了核酸提取,扩增和检测功能,可以在集成的检测设备中自动执行。这种新颖的诊断工具显示出良好的检测限,稳定性(变异系数<6%),特异性(每个靶标均不与其他23种病原体发生交叉反应)以及对其他物质的抗性,其诊断功效与PCR相似。从样品到自动测试步骤的响应时间缩短到50分钟。这种新颖的诊断工具具有高度集成,自动化,从样品到答案的检测等优点,因此可以替代传统方法。

更新日期:2020-08-03
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