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Detoxification roles of tributyltin-binding protein type 2 in Japanese medaka (Oryzias latipes) exposed to tributyltin.
Marine Pollution Bulletin ( IF 5.8 ) Pub Date : 2020-08-03 , DOI: 10.1016/j.marpolbul.2020.111445
Xuchun Qiu 1 , Takumi Takamura 2 , Shintaro Enoki 3 , Yoko Kato-Unoki 4 , Yuki Takai 3 , Yosuke Nagano 3 , Masato Kinoshita 5 , Takeshi Kitano 6 , Yohei Shimasaki 3 , Yuji Oshima 7
Affiliation  

Tributyltin-binding protein type 2 (TBT-bp2), a homolog of α1-acid glycoprotein, may contribute to both accumulation and detoxification of TBT in fish. In this study, we conducted acute TBT exposure tests using both wide-type (WT) and TBT-bp2−/− (KO) strains of medaka and compared their responses in survival time and accumulation of TBT. Deficiency of TBT-bp2 significantly accelerated the time to death of medaka and decreased the LC50 of TBT, indicating that the KO-strain is more sensitive to TBT. No significant difference in the intrinsic TBT concentration in surviving fish was observed between the two strains. However, the intrinsic TBT concentration in dead KO-strain was significantly lower than that in WT-strain. These findings provide direct evidence, supporting the hypothesis that TBT-bp2 plays a critical role in the detoxification of TBT in fish.



中文翻译:

三丁基锡结合蛋白 2 型在日本青鳉 (Oryzias latipes) 中的解毒作用。

三丁基锡结合蛋白 2 型 (TBT-bp2) 是 α1-酸性糖蛋白的同源物,可能有助于 TBT 在鱼体内的积累和解毒。在这项研究中,我们使用青鳉的宽型 (WT) 和 TBT-bp2 -/- (KO) 菌株进行了急性 TBT 暴露测试,并比较了它们在存活时间和 TBT 积累方面的反应。TBT-bp2 的缺乏显着加快了青鳉的死亡时间并降低了 LC 50TBT,表明KO菌株对TBT更敏感。在两种菌株之间未观察到存活鱼的内在 TBT 浓度有显着差异。然而,死 KO 菌株的内在 TBT 浓度显着低于 WT 菌株。这些发现提供了直接证据,支持 TBT-bp2 在鱼类 TBT 解毒中起关键作用的假设。

更新日期:2020-08-03
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