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Ultrasound-assisted modified pectin from unripe fruit pomace of raspberry (Rubus chingii Hu): Structural characterization and antioxidant activities
LWT - Food Science and Technology ( IF 6 ) Pub Date : 2020-08-03 , DOI: 10.1016/j.lwt.2020.110007
Yue Chen , Yajie Wang , Leilei Xu , Yanan Jia , Zihan Xue , Min Zhang , Muenduen Phisalaphong , Haixia Chen

With the aim of making full use of the resource of Rubus Chingii Hu, this work comparatively investigated the structural characterization and antioxidant activities of pectin (RP) from unripe fruit pomaces of Rubus chingii Hu and ultrasonic-modified pectin (URP) from unripe fruit pomaces of Rubus Chingii Hu. Compared with the original RP, ultrasound treated RP (URP) significantly changed the chemical composition. The scanning electron microscopy (SEM) results suggested that the conformation of RP was filiform, smooth and slim and a large number of spherical pectins were produced by ultrasonic modification. Both RP and URP exhibited the potential antioxidant activity and α-glucosidase inhibitory activity. Moreover, RP and URP effectively attenuated the oxidative damage induced by H2O2 and reduced intracellular reactive oxygen species (ROS) levels in human hepatic L02 cells and URP showed higher activity than RP. These results suggested that the unripe fruit pomace of Rubus chingii Hu could be used as a potential resource to obtain the antioxidant pectin and the ultrasound-assisted modification could significantly change the physical structure of pectin and effectively improve its antioxidant activities.



中文翻译:

超声对未成熟果树莓果胶(Rubus chingii Hu)果胶的改性:结构表征和抗氧化活性

为了充分利用中药悬钩子的资源,本研究比较了中成药悬钩子的未成熟果实果胶和未经修饰的果肉的超声波改性果胶(URP)的结构特征和抗氧化活性。的悬天目胡。与原始RP相比,超声处理的RP(URP)显着改变了化学成分。扫描电镜(SEM)结果表明,RP的构型为丝状,光滑且细小,通过超声改性可制得大量球形果胶。RP和URP均表现出潜在的抗氧化活性和α-葡萄糖苷酶抑制活性。此外,RP和URP可有效减轻H 2 O 2诱导的氧化损伤,并降低人肝L02细胞中的细胞内活性氧(ROS)水平,且URP的活性高于RP。这些结果表明悬钩子未成熟的果渣 胡可作为获得抗氧化剂果胶的潜在资源,超声辅助修饰可显着改变果胶的物理结构,有效提高其抗氧化活性。

更新日期:2020-08-18
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