Hydrogels are receiving increasing attention in bioapplications. Among hydrogels, calcium alginate (Ca-alginate) hydrogels are widely used for their biocompatibility, low toxicity, low cost, and rapid fabrication by simple mixing of Ca²⁺ and sodium alginate (Na-alginate). For bioapplications using hydrogels, it is necessary to construct designed hydrogel structures. Although several methods have been proposed for fabricating designed hydrogels, a simple and low-cost method is desirable. Therefore, we developed a new method using sacrificial templates of sugar structures to fabricate three-dimensional (3D) designed Ca-alginate hydrogels. In this method, Na-alginate solution is mixed with molten sugar, and the resulting highly viscous material used to mold 3D sugar structures as sacrificial templates. Since sugar constructs are easily handled compared to hydrogels, sugar templates are useful for preparing 3D constructs. Finally, the sugar and Na-alginate structure is immersed in a CaCl₂ solution to simultaneously dissolve the template and form the Ca-alginate hydrogel. The resulting hydrogel takes the shape of the sugar template. By stacking and fusing various sugar structures, such as fibers and blocks, 3D designed Ca-alginate hydrogels can be successfully fabricated. This simple and low-cost method shows excellent potential for application to a variety of bioapplications.

水凝胶在生物应用中越来越受到关注。在水凝胶中，海藻酸钙（Ca-alginate）水凝胶因其生物相容性，低毒性，低成本以及通过简单地混合Ca ²⁺而快速制备而被广泛使用。和海藻酸钠（Na-alginate）。对于使用水凝胶的生物应用，必须构建设计的水凝胶结构。尽管已经提出了几种方法来制造设计的水凝胶，但是期望一种简单且低成本的方法。因此，我们开发了一种新的方法，使用糖结构的牺牲模板来制作三维（3D）设计的钙藻酸盐水凝胶。在此方法中，将海藻酸钠溶液与熔融糖混合，然后将所得的高粘度材料用于成型3D糖结构作为牺牲模板。由于与水凝胶相比，糖构建体易于处理，因此糖模板可用于制备3D构建体。最后，将糖和藻酸钠结构浸入CaCl _2中溶液同时溶解模板并形成海藻酸钙水凝胶。所得的水凝胶呈糖模板的形状。通过堆叠和融合各种糖结构（例如纤维和块），可以成功制造3D设计的Ca-藻酸盐水凝胶。这种简单且低成本的方法显示出将其应用于多种生物应用的巨大潜力。