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Innate immune function of serine/threonine-protein kinase from Macrobrachium rosenbergii in response to host-pathogen interactions.
Fish & Shellfish Immunology ( IF 4.7 ) Pub Date : 2020-08-03 , DOI: 10.1016/j.fsi.2020.07.068
Gayathri Ravichandran 1 , Mukesh Pasupuleti 2 , Mariadhas Valan Arasu 3 , Naif Abdullah Al-Dhabi 3 , Aziz Arshad 4 , Jesu Arockiaraj 1
Affiliation  

The occurrences of multiple drug-resistant strains have been relentlessly increasing in recent years. The aquaculture industry has encountered major disease outbreaks and crucially affected by this situation. The usage of non-specific chemicals and antibiotics expedites the stimulation of resistant strains. Triggering the natural defense mechanism would provide an effective and safest way of protecting the host system. Hence, we have investigated the innate immune function of serine/threonine-protein kinase (STPK) in Macrobrachium rosenbergii (Mr). The in-silico protein analysis resulted in the identification of cationic antimicrobial peptide, MrSL-19, with interesting properties from STPK of M. rosenbergii. Antimicrobial assay, FACS and SEM analysis demonstrated that the peptide potentially inhibits Staphylococcus aureus by interacting with its membrane. The toxic study on MrSL-19 demonstrated that the peptide is not toxic against HEK293 cells as well as human erythrocytes. This investigation showed the significant innate immune property of an efficient cationic antimicrobial peptide, MrSL-19 of STPK from M. rosenbergii.



中文翻译:

罗氏沼虾丝氨酸/苏氨酸蛋白激酶对宿主-病原体相互作用的先天免疫功能。

近年来,多重耐药菌株的出现一直在持续增加。水产养殖业遇到了重大疾病暴发,并受到这种情况的严重影响。非特异性化学药品和抗生素的使用可加快抗药性菌株的刺激。触发自然防御机制将提供保护主机系统的有效和最安全的方法。因此,我们研究了罗氏沼虾中丝氨酸/苏氨酸蛋白激酶(STPK)的先天免疫功能(Mr)。的在计算机芯片蛋白分析导致阳离子抗微生物肽的鉴定,先生SL-19,与来自的STPK有趣的性质罗氏沼虾。抗菌测定,FACS和SEM分析表明,该肽可能通过与膜相互作用而抑制金黄色葡萄球菌。对SL-19先生的毒性研究表明,该肽对HEK293细胞和人类红细胞均无毒。该研究表明,有效的阳离子抗菌肽罗氏沼虾STPK SL-19先生具有显着的先天免疫特性

更新日期:2020-08-19
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