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Stenotrophomonas cyclobalanopsidis sp. nov., isolated from the leaf spot disease of Cyclobalanopsis patelliformis.
Antonie van Leeuwenhoek ( IF 2.6 ) Pub Date : 2020-08-03 , DOI: 10.1007/s10482-020-01453-y
Dan-Ran Bian 1 , Han Xue 1 , Chun-Gen Piao 1 , Yong Li 1
Affiliation  

A Gram-negative, facultatively anaerobic, motile bacterial strain, TPQG1-4T, was isolated from the leaf of Cyclobalanopsis patelliformis with spot disease. The isolate was investigated using the polyphasic taxonomic approach. 16S rRNA gene sequencing and analyzing revealed that the novel strain shares the highest sequence similarity with Stenotrophomonas lactitubi M15T (99.6%), Stenotrophomonas indicatrix WS40T (99.4%), Stenotrophomonas maltophilia IAM 12423T (99.2%) and Stenotrophomonas pavanii LMG 25348T (99.0%). In phylogenetic trees based on 16S rRNA gene sequences, the novel strain branched independently from other species of Stenotrophomonas. Average nucleotide identity values between the novel isolate and S. lactitubi M15T, S. indicatrix WS40T, S. maltophilia IAM 12423T, S. pavanii LMG 25348T, and Pseudomonas geniculata ATCC 19374T were 87.2%, 87.3%, 86.3%, 88.0%, and 81.3%, respectively, suggesting the isolate was a novel species of the genus Stenotrophomonas. The DNA G + C content of TPQG1-4T is 67.1 mol%. The major fatty acids were iso-C15:0 (25.4%) and anteiso-C15:0 (17.0%). The polar lipids of TPQG1-4T included phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, amino phospholipid and phospholipid. Based on phenotypic and genotypic characteristics, the strain represents a novel species in the genus Stenotrophomonas, for which the name Stenotrophomonas cyclobalanopsidis sp. nov. is proposed. The type strain is TPQG1-4T (= CFCC 15341T = LMG 31208T).



中文翻译:

嗜麦芽单胞菌 nov。,分离自巴氏青冈叶斑病。

革兰氏阴性,兼性厌氧,能动细菌菌株,TPQG1-4 Ť,从叶分离青冈栎与斑病。使用多相分类学方法对分离物进行了研究。16S rRNA基因测序和分析,发现该新菌株股具有最高的序列相似性嗜lactitubi M15 Ť(99.6%),嗜椭WS40 Ť(99.4%),嗜麦芽窄食IAM 12423 Ť(99.2%)和嗜pavanii LMG 25348 Ť(99.0%)。在基于16S rRNA基因序列的系统树中,该新菌株独立于嗜麦芽单胞菌的其他物种分支新颖的分离物和之间的平均核苷酸同一性值S. lactitubi M15 ŤS.椭WS40 ŤS.麦芽IAM 12423 ŤS. pavanii LMG 25348 Ť,和假单胞菌膝ATCC 19374 Ť分别为87.2%,87.3%,86.3% ,分别为88.0%和81.3%,表明该分离物是嗜麦芽单胞菌属的一种新物种TPQG1-4 T的DNA G + C含量为67.1摩尔%。主要脂肪酸是异-C 15:0(25.4%)和对异-C 15:0(17.0%)。TPQG1-4的极性脂质牛逼包括磷脂酰乙醇胺,磷脂酰甘油,二磷脂酰甘油,磷脂氨基酸和磷脂。基于表型和基因型特征,该菌株代表了麦芽单胞菌属中的一个新物种,其名称为嗜麦芽单胞cyclobalanopsidis sp。十一月 被提议。类型菌株为TPQG1-4 T(= CFCC 15341 T  = LMG 31208 T)。

更新日期:2020-08-03
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