Depending on the culture conditions, Euglena gracilis can produce a variety of macromolecules such as vitamins, lipids, proteins, and polysaccharides. In particular, the ability of E. gracilis to store a lot of β-glucans implies it has significant biotechnological potential. In this study, the growth of E. gracilis and its paramylon content were improved through co-cultivation with the bacterium Pseudoalteromonas sp. MEBiC 03607. To determine the optimal co-cultivation strategy, the effects of the algae-to-bacterial inoculum ratio and growth stage of E. gracilis were examined. Under optimal conditions, the growth of E. gracilis and its paramylon content were increased by more than 17 and 25%, respectively, as a direct result of the cultivation with bacteria. Through qRT-PCR analysis, it was confirmed that when the bacteria were inoculated, the expression of gene related to β-glucan synthase was increased, while the expression of wax ester synthase-related gene was decreased. This indicates that bacteria promote the synthesis of β-glucans of E. gracilis and inhibit the conversion of β-glucan to wax ester. These results demonstrate a novel approach to improve the growth of E. gracilis and its paramylon productivity.

视培养条件而定，Euglena gracilis可以产生多种大分子，例如维生素，脂质，蛋白质和多糖。特别地，细粒肠杆菌存储大量β-葡聚糖的能力暗示其具有显着的生物技术潜力。在这项研究中，生长眼虫和其副淀粉含量是通过共培养用细菌提高假交替单胞菌属。MEBiC 03607.为了确定最佳的共培养策略，藻类对细菌接种物比率和生长阶段的影响小眼虫进行了研究。在最佳条件下，细叶肠球菌的生长细菌培养的直接结果是，其副甲的含量分别增加了17％和25％以上。通过qRT-PCR分析证实，接种细菌时，与β-葡聚糖合酶相关的基因的表达增加，而与蜡酯合酶相关的基因的表达减少。这表明细菌促进的β葡聚糖的合成小眼虫并抑制β-D-葡聚糖，以蜡酯的转化。这些结果表明了一种新方法，以提高的生长小眼虫和其副淀粉生产率。