Two new lectins from the green alga Codium isthmocladum (CiL-1 and CiL-2) were isolated. Both lectins could agglutinate human and rabbit erythrocytes. Galactosides and fetuin showed inhibitory effect on CiL-1. CiL-2 was inhibited by GalNAc and porcine stomach mucin. CiL-1 was a monomeric protein of 12 kDa, whereas CiL-2 showed 12 kDa in SDS-PAGE and an oligomeric state in gel filtration. MALDI-ToF-MS of CiL-1 revealed a molecular mass of 12.027 ± 5 Da, while CiL-2 showed molecular mass of 12.264 ± 5 Da. Ninety-eight percent of CiL-1’s primary structure was determined consisting of 112 residues placed in two repeated domains with approximately 60% of similarity. CiL-1 showed similarity with hypothetical proteins from aquatic pathogenic fungi. The N-terminal of CiL-2 showed no similarity to CiL-1 or to any known protein. The three-dimensional model of CiL-1 consists of four two-strand β-sheets disposed in a barrel-like arrangement, connected by loops of variable sizes, with a well-structured hydrophobic core. Binding site prediction suggests the existence of two independent monosaccharide binding sites in CiL-1. The lectins showed no antibacterial activity on Gram-positive and Gram-negative bacteria, but they were able to significantly inhibit the biofilm formation from Staphylococcus aureus and Staphylococcus epidermidis.

从绿藻两个新的外源凝集素刺松isthmocladum（CiL-1和CiL-2）被分离。两种凝集素均可凝集人和兔的红细胞。半乳糖苷和胎球蛋白对CiL-1具有抑制作用。CiL-2被GalNAc和猪胃粘蛋白抑制。CiL-1是12 kDa的单体蛋白，而CiL-2在SDS-PAGE中显示12 kDa，在凝胶过滤中呈寡聚状态。CiL-1的MALDI-ToF-MS显示分子量为12.027±5 Da，而CiL-2显示的分子量为12.264±5 Da。确定了98％的CiL-1一级结构，由位于两个重复域中的112个残基组成，具有大约60％的相似性。CiL-1与水生病原性真菌的假定蛋白表现出相似性。CiL-2的N端显示与CiL-1或任何已知蛋白无相似性。CiL-1的三维模型由四个呈桶状排列的两链β-折叠片组成，通过可变大小的环连接，并具有结构良好的疏水核。结合位点预测表明CiL-1中存在两个独立的单糖结合位点。凝集素对革兰氏阳性和革兰氏阴性细菌均未显示抗菌活性，但它们能够显着抑制细菌的生物膜形成。金黄色葡萄球菌和表皮葡萄球菌。