In this study, simultaneous removal of ammonium plus phenol could be achieved by Alcaligenes faecalis strain WY-01 with the addition of acetate, although acetate delayed the phenol degradation, probably due to the delayed expression of phenol hydroxylase gene under the presence of acetate. Moreover, the successful expression of key enzyme genes in strain WY-01 provided some evidence to illustrate its metabolic pathways of ammonium and phenol under aerobic conditions. Furthermore, SEM was used to clarify the role of acetate in resisting phenol toxicity, and these results demonstrated that strain WY-01 has the ability to form cell flocs when sodium acetate is used as co-substrate for a high concentration of phenol, and these flocs could protect cells against the toxicity of phenol, further enhancing phenol degradation in a high concentration of phenol. All these will provide further insights into the efficacy of strain WY-01 for treating wastewater cocontaminated by ammonium and phenol.

本研究中，粪产碱菌WY-01 菌株加入醋酸盐可以同时去除铵盐和苯酚，尽管醋酸盐延迟了苯酚的降解，可能是由于苯酚羟化酶基因的延迟表达在醋酸盐存在下。此外，菌株WY-01中关键酶基因的成功表达为说明其在有氧条件下的铵和苯酚代谢途径提供了一些证据。此外，SEM还用于阐明乙酸盐在抗酚毒性中的作用，这些结果表明，当乙酸钠作为高浓度苯酚的共底物时，菌株WY-01具有形成细胞絮凝物的能力，并且这些絮凝物可以保护细胞免受苯酚的毒性，在高浓度苯酚中进一步增强苯酚降解。所有这些都将进一步深入了解菌株 WY-01 处理铵和苯酚共污染废水的功效。