In this study, the biohydrogen production ability of isolated strains with sago industrial effluent in anaerobic batch fermentation was investigated. The bacteria responsible for the biohydrogen were isolated and identified as Clostridium sartagoforme NASGE 01 and Enterobacter cloacae NASGE 02. The volume of biohydrogen gas generated from the effluent was determined by gas chromatography (GC) and the organic acids formed during the biohydrogen production were determined by GC equipped with a flame ionization detector (GC—FID). In batch fermentation, C. sartagoforme NASGE 01 produced high amount of biogas (232 ± 11.02 mL/L) and biohydrogen (41.5%) followed by E. cloacae NASGE 02 produced 212.8 ± 8 mL/L biogas containing 31.5% of biohydrogen. Moreover, the hydrogen production potential (P), production rate (Rm) and lag time ( λ ) were analyzed from Gompertz non-linear curve fit model. The peak hydrogen yield was obtained with C. sartagoforme NASGE 01 was 158.7 mL/g glucose (1.26 mol H 2 /mol glucose) with the substrate degradation of 56.7%. Butyric acid was the major organic acid formed while hydrogen production with Clostridium sartagoforme NASGE 01 (176.4 mg/L) and Enterobacter cloacae NASGE 02 (285.1 mg/L). These experimental data demonstrated the feasibility of biohydrogen production using pure culture of anaerobic bacteria with sago industrial waste water as substrate.

中文翻译：

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从西米工业废水中分离的产氢气厌氧菌的鉴定

本研究考察了西米工业废水分离菌株在厌氧分批发酵中的产氢能力。分离出产生生物氢气的细菌并鉴定为 Clostridium sartagoforme NASGE 01 和 Enterobacter cloacae NASGE 02。通过气相色谱法 (GC) 确定流出物中产生的生物氢气的体积，并且在生物氢气生产过程中形成的有机酸由下式确定配备火焰离子化检测器 (GC-FID) 的 GC。在分批发酵中，C. sartagoforme NASGE 01 产生大量沼气 (232 ± 11.02 mL/L) 和生物氢 (41.5%)，其次是 E. cloacae NASGE 02 产生了 212.8 ± 8 mL/L 的沼气，其中含有 31.5% 的生物氢。此外，产氢潜力（P），从 Gompertz 非线性曲线拟合模型分析生产率 (Rm) 和滞后时间 (λ)。用 C. sartagoforme NASGE 01 获得的峰值氢产量为 158.7 mL/g 葡萄糖（1.26 mol H 2 /mol 葡萄糖），底物降解率为 56.7%。丁酸是沙塔哥形梭菌 NASGE 01 (176.4 mg/L) 和阴沟肠杆菌 NASGE 02 (285.1 mg/L) 制氢时形成的主要有机酸。这些实验数据证明了以西米工业废水为底物的厌氧菌纯培养物生产生物制氢的可行性。丁酸是沙塔哥形梭菌 NASGE 01 (176.4 mg/L) 和阴沟肠杆菌 NASGE 02 (285.1 mg/L) 制氢时形成的主要有机酸。这些实验数据证明了以西米工业废水为底物的厌氧菌纯培养物生产生物制氢的可行性。丁酸是沙塔哥形梭菌 NASGE 01 (176.4 mg/L) 和阴沟肠杆菌 NASGE 02 (285.1 mg/L) 制氢时形成的主要有机酸。这些实验数据证明了以西米工业废水为底物的厌氧菌纯培养物生产生物制氢的可行性。