Tomato leaf mould disease is a serious foliar disease caused by Cladosporium fulvum, resulting in a significant reduction in yield and quality, especially under protected cultivation. Finding new resistant genes against leaf mould has become one of the important goals of tomato breeding. Cf‐12‐tomato showed good resistance to several C. fulvum races. The Cf‐12 was mapped using re‐sequencing and BSA methods. Allelism test for Cf‐12, Cf‐5 and Cf‐9 was performed. The results showed that Cf‐12 is an independently assigned C. fulvum resistance gene. About 67,853 high‐quality polymorphic SNPs were found and 233 genes were annotated in three associated regions of 5.85 Mb on Chr. 6s. Three genes with typical Cf‐type characters encoding the leucine‐rich repeat receptor‐like protein were screened out. Using qRT‐PCR, structural and phylogenetic analysis, only the Solyc06g008265.1.1‐CGN7495 was finally identified. An effective InDel marker, InD‐3F/1R, was designed for MAS breeding. Our research provides new insights into the mapping of the Cf‐12, and provides a basis for Cf‐12 cloning and the Cf genetic evolution analysis.

中文翻译：

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高通量测序技术对番茄（Cola-12 lycopersicum）枝叶孢霉抗性基因Cf-12进行定位和候选基因筛选

番茄叶片霉菌病是一种由黄叶Cladosporium fulvum引起的严重叶病，导致产量和质量显着下降，尤其是在受保护的栽培条件下。寻找新的抗叶霉病抗性基因已成为番茄育种的重要目标之一。CF-12 -tomato显示出几个不错的阻力C.黄枝孢霉的比赛。的CF-12，使用重测序和BSA方法映射。对Cf-12，Cf-5和Cf-9进行了等位性测试。结果表明Cf-12是一个独立分配的C.fulvum抗性基因。在Chr上的三个相关区域（5.85 Mb）中发现了约67,853个高质量多态性SNP，并注释了233个基因。6s。筛选出三个具有典型Cf型特征的基因，它们编码富含亮氨酸的重复受体样蛋白。使用qRT-PCR，结构和系统发育分析，仅最终鉴定出Solyc06g008265.1.1- CGN7495。有效的InDel标记InD-3F / 1R是为MAS育种设计的。我们的研究为Cf-12的映射提供了新的见解，并为Cf-12克隆和Cf遗传进化分析提供了基础。