T-2 toxin is the most toxic as a type A trichothecenes, which could contaminate grains, especially in wheat and corn. It can cause immune suppression, neurotoxicity, the apoptosis of cells and even induce tumorigenesis. Recent studies have indicated that selenium (Se) have protective effect against mycotoxins-induced toxicity. The present studies was designed to investigate the protective role of Selenomethionine (SeMet) on T-2 toxin-induced toxicity in rabbit's jejunum. 50 New Zealand rabbits were divided into five group (Control group, T-2 group, low-dose Se + T-2 group, medium-dose + T-2 group and high-dose Se + T-2 group). New Zealand rabbits were orally administered with SeMet (0.2, 0.4 and 0.6 mg/kg, Adding diet) for 21 days. On 17th days, each group began to take 0.4 mg/kg of T-2 toxin orally every day for 5 days. We found that rabbit exposed to T-2 toxin could increase the levels of ROS, and decrease activities of antioxidant enzymes and the expression of Occludin and ZO-1. In addition, T-2 toxin could trigger jejunal inflammatory response and enhance the expression of IL-1β, IL-6 and TNF-α. After SeMet pretreatment, our results indicated that Se attenuated the T-2 toxin-induced oxidative stress, decreasing the level of ROS, MDA and enhancing the activity of SOD and GSH-Px. Moreover, SeMet can alleviate jejunal inflammatory response, and protect the integrity of the intestinal barrier through up-regulating the expression of ZO-1 and Occludin. In the present research, supplementation of 0.2 mg/kg SeMet in the diet could effectively alleviate the T-2 toxin poisoning in rabbits.

T-2毒素作为A型毛癣菌毒素最具毒性，可能污染谷物，尤其是小麦和玉米。它可以引起免疫抑制，神经毒性，细胞凋亡，甚至诱发肿瘤发生。最近的研究表明硒对霉菌毒素诱导的毒性具有保护作用。本研究旨在研究硒蛋氨酸（SeMet）对兔空肠中T-2毒素诱导的毒性的保护作用。将50只新西兰大白兔分为5组（对照组，T-2组，低剂量Se + T-2组，中剂量+ T-2组和高剂量Se + T-2组）。新西兰兔子口服SeMet（0.2、0.4和0.6 mg / kg，添加饮食）21天。在17日天，每组开始每天口服0.4 mg / kg的T-2毒素，持续5天。我们发现，暴露于T-2毒素的家兔可以增加ROS的水平，并降低抗氧化酶的活性以及Occludin和ZO-1的表达。此外，T-2毒素可引发空肠炎症反应并增强IL-1β，IL-6和TNF-α的表达。之后蛋氨酸预处理，我们的研究结果表明，硒衰减的T-2毒素诱导的氧化应激，降低ROS，MDA含量，提高SOD和GSH-Px活性。此外，SeMet可以通过上调ZO-1和Occludin的表达来减轻空肠的炎症反应，并保护肠道屏障的完整性。在本研究中，补充量为0.2 mg / kg日粮中的SeMet可以有效减轻兔子的T-2毒素中毒。