The production of secondary metabolites in the submerged co-cultures of Penicillium rubens Wisconsin 54-1255 and Aspergillus terreus ATCC 20542 was evaluated. The biosynthetic capabilities of the two strains were compared in a set of diverse liquid media that differed with respect to the initial levels of glucose, lactose and yeast extract, contained carrot juice or vegetable/turkey puree as additional nutrient sources or were supplemented with phenylacetic acid, the side-chain precursor of penicillin G. The main goal of the study was to investigate the interactions between A. terreus and P. rubens that might contribute to the changes of secondary metabolite titers. Briefly, the biosynthesis of octaketide metabolites (+)-geodin and asterric acid was visibly enhanced as a result of replacing the conventional monocultures with the co-culture systems, but solely in the media containing not more than 5 g L⁻¹ of yeast extract. By contrast, no marked enhancement was observed with respect to the biosynthesis of penicillin G, lovastatin, chrysogine, 4a,5-dihydromevinolinic acid and 3α-hydroxy-3,5-dihydromonacolin L acid. It was shown that the relationships between medium composition and product titers were clearly different in monoculture variants than in the corresponding co-cultures. Finally, it was demonstrated that the utilization of penicillin precursors by P. rubens can be blocked under the conditions of co-cultivation.

评估了深青霉威斯康星州54-1255和土曲霉ATCC 20542的水下共培养物中次生代谢产物的产生。在一组不同的液体培养基中比较了这两种菌株的生物合成能力，这些培养基在葡萄糖，乳糖和酵母提取物的初始水平方面有所不同，包含胡萝卜汁或蔬菜/火鸡泥作为其他营养源或补充了苯乙酸。 ，是青霉素G的侧链前体。该研究的主要目的是研究土壤曲霉和红假单胞菌之间的相互作用可能有助于次级代谢产物滴度的变化。简而言之，由于用共培养系统替代了传统的单培养物，因此明显增强了octaketide代谢产物（+）-geodin和星状酸的生物合成，但仅在含有不超过5 g L ^-1酵母提取物的培养基中。相比之下，未观察到关于青霉素G，洛伐他汀，金霉素，4a，5-二氢甲基萘酸和3α-羟基-3,5-二氢莫纳可林L酸的生物合成的显着增强。结果表明，单培养变体中培养基组成与产物滴度之间的关系明显不同于相应的共培养。最后，证明了红假单胞菌对青霉素前体的利用 在共同培养的条件下可以被封锁。