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Transcriptomic analysis of Manila clam Ruditapes philippinarum under lipopolysaccharide challenge provides molecular insights into immune response.
Fish & Shellfish Immunology ( IF 4.7 ) Pub Date : 2020-08-02 , DOI: 10.1016/j.fsi.2020.07.022
Shuqi Zuo 1 , Kunyin Jiang 1 , Dongdong Li 1 , Xiwu Yan 1 , Hongtao Nie 1
Affiliation  

The Manila clam, Ruditapes philippinarum, is an economically important shellfish in marine aquaculture. A better understanding of the immune system in R. philippinarum will provide the basis for the development of strategies to mitigate the impact of infectious diseases affecting this species but can also be of relevance for other bivalves of commercial interest. In this study, the transcriptional response of the Manila clam under lipopolysaccharide (LPS) challenge was characterized using RNA sequencing. The transcriptomes of LPS challenged group of clams (LH1, LH2 and LH3), and the PBS control group (CH1, CH2 and CH3), were sequenced with the Illumina HiSeq platform. Compared with the unigene expression profile of the control group, 223 unigenes were up-regulated and 389 unigenes were down-regulated in the LPS challenged group. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses revealed that signal transduction, defense response, and immune-related pathways such as Chemokine signaling pathway, Complement and coagulation cascades, NOD-like receptor signaling pathway, and Inflammatory mediator regulation of TRP channels in sensory system were the most highly enriched pathways among the genes that were differentially expressed under LPS challenge. This study present understanding of the molecular basis underpinning response to LPS challenge and provides useful information for future work on the molecular mechanism of pathogen resistance and immunity in Manila clam.



中文翻译:

脂多糖挑战下马尼拉蛤仔菲律宾蛤的转录组学分析提供了免疫应答的分子见解。

马尼拉蛤,菲律宾蛤仔(Ruditapes philippinarum),是海水养殖中经济上重要的贝类。更好地了解菲律宾蛤仔的免疫系统将为制定战略以减轻影响该物种的传染病的影响提供基础,但也可能与其他具有商业意义的双壳类动物有关。在这项研究中,使用RNA测序表征了在脂多糖(LPS)攻击下马尼拉蛤的转录反应。用Illumina HiSeq平台对蛤的LPS攻击组(LH1,LH2和LH3)和PBS对照组(CH1,CH2和CH3)的转录组进行测序。与对照组的单基因表达谱相比,LPS攻击组的223个单基因上调,而389个单基因下调。基因本体论(GO)和《京都基因与基因组百科全书》(KEGG)分析表明,信号转导,防御反应,在LPS刺激下差异表达的基因中,趋化因子信号转导途径,补体和凝血级联反应,NOD样受体信号转导途径以及炎症介质调节TRP通道等免疫相关途径是最丰富的途径。这项研究提出了对脂多糖挑战的分子基础的理解,并为今后研究马尼拉蛤的病原体抗性和免疫性的分子机制提供了有用的信息。

更新日期:2020-08-12
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