Kidney regeneration could be classified into 2 groups: kidney generation and kidney repair. We have attempted in vivo nephron generation for kidney repair, as a therapy for chronic renal failure (CRF), by exploiting cellular interactions via conditioned media. In the previous report, we demonstrated the generation of rich nephrons in rat intact kidney cortices through percapsular injection of mesenchymal stem cell (MSC)-differentiated tubular epithelial cells (TECs) after pretreatment of 3-dimensional culture using a small amount of gel complex and condensed medium. In this study, to verify the amelioration of serum creatinine (sCr) levels by regenerated nephrons in rats with CRF, we first created damaged kidneys through systemic administration of adriamycin, and implanted the pretreated MSC-differentiated TECs into unilateral kidney cortices 2 weeks after adriamycin administration (A-2W, that is I–0W). After recovery of acute kidney injury, the control rats without cell implantation showed re-exacerbation of sCr levels, resulting in death within A-12W. Alternatively, the cell-implanted rats had a formation of mature nephrons in I–3W, and showed significant amelioration of sCr levels in I–7W. As a result, these rats could live until euthanization in I–12W or I–16W, indicating the utility of cell injection therapy into a kidney (K-CIT) for CRF. We expect that our K-CIT or the refined methods will be applied to patients with CRF.

肾脏再生可分为两组：肾脏生成和肾脏修复。我们已经尝试了体内通过利用条件培养基的细胞相互作用，生成用于肾脏修复的肾单位，作为慢性肾衰竭（CRF）的疗法。在先前的报告中，我们证明了在使用少量凝胶复合物预处理3维培养物后，通过经间充质干细胞（MSC）分化的肾小管上皮细胞（TECs）的包囊注射，在大鼠完整的肾皮质中生成丰富的肾单位。浓缩介质。在这项研究中，为了验证CRF大鼠中再生肾单位对血清肌酐（sCr）水平的改善，我们首先通过全身性给予阿霉素来制造受损的肾脏，并将经过预处理的MSC分化的TECs植入阿霉素2周后的单侧肾皮质中。管理（A-2W，即I-0W）。急性肾损伤恢复后，没有细胞植入的对照大鼠显示sCr水平再次加重，导致A-12W内死亡。另外，植入细胞的大鼠在I–3W中形成了成熟的肾单位，并在I–7W中显示出sCr水平的显着改善。结果，这些大鼠可以活到I-12W或I-16W安乐死为止，这表明向CRF的肾脏（K-CIT）细胞注射疗法的实用性。我们希望我们的K-CIT或改进的方法将适用于CRF患者。表明细胞注射疗法可用于CRF的肾脏（K-CIT）。我们希望我们的K-CIT或改进的方法将适用于CRF患者。表明细胞注射疗法可用于CRF的肾脏（K-CIT）。我们希望我们的K-CIT或改进的方法将适用于CRF患者。