The xenoestrogenic mycotoxin zearalenone is a Fusarium-derived food and feed contaminant. In mammals, the reduced (e.g., zearalanone, α-zearalanol, and β-zearalanol) and conjugated (e.g., zearalenone-14-sulfate) metabolites of zearalenone are formed. Furthermore, filamentous fungi and plants are also able to convert zearalenone to conjugated derivatives, including zearalenone-14-sulfate and zearalenone-14-glucoside, respectively. Serum albumin is the dominant plasma protein in the circulation; it interacts with certain mycotoxins, affecting their toxicokinetics. In a previous investigation, we demonstrated the remarkable species differences regarding the albumin binding of zearalenone and zearalenols. In the current study, the interactions of zearalanone, α-zearalanol, β-zearalanol, zearalenone-14-sulfate, and zearalenone-14-glucoside with human, bovine, porcine, and rat serum albumins were examined, employing fluorescence spectroscopy and affinity chromatography. Zearalanone, zearalanols, and zearalenone-14-sulfate form stable complexes with albumins tested (K = 9.3 × 10³ to 8.5 × 10⁵ L/mol), while the albumin binding of zearalenone-14-glucoside seems to be weak. Zearalenone-14-sulfate formed the most stable complexes with albumins examined. Considerable species differences were observed in the albumin binding of zearalenone metabolites, which may have a role in the interspecies differences regarding the toxicity of zearalenone.

异雌激素霉菌毒素玉米赤霉烯酮是一种源自镰刀菌的食品和饲料污染物。在哺乳动物中，形成玉米赤霉烯酮的还原代谢物（例如，玉米赤霉酮、α-玉米赤霉醇和β-玉米赤霉醇）和缀合代谢物（例如，玉米赤霉烯酮-14-硫酸酯）。此外，丝状真菌和植物还能够将玉米赤霉烯酮转化为共轭衍生物，分别包括玉米赤霉烯酮-14-硫酸盐和玉米赤霉烯酮-14-葡萄糖苷。血清白蛋白是循环中主要的血浆蛋白；它与某些霉菌毒素相互作用，影响其毒代动力学。在之前的研究中，我们证明了玉米赤霉烯酮和玉米赤霉烯醇的白蛋白结合方面存在显着的物种差异。在当前的研究中，采用荧光光谱和亲和色谱法检测了玉米赤霉酮、α-玉米赤霉醇、β-玉米赤霉醇、玉米赤霉烯酮-14-硫酸盐和玉米赤霉烯酮-14-葡萄糖苷与人、牛、猪和大鼠血清白蛋白的相互作用。 。玉米赤霉酮、玉米赤霉醇和玉米赤霉烯酮 14 硫酸盐与测试的白蛋白形成稳定的复合物（K = 9.3 × 10 ³至 8.5 × 10 ⁵ L/mol），而玉米赤霉烯酮 14 葡萄糖苷的白蛋白结合似乎很弱。玉米赤霉烯酮-14-硫酸盐与所检测的白蛋白形成最稳定的复合物。在玉米赤霉烯酮代谢物的白蛋白结合中观察到相当大的物种差异，这可能在玉米赤霉烯酮毒性的种间差异中发挥作用。