This study investigated for the first time the production of β-glucosidase from Penicillium roqueforti ATCC 10110 by solid-state fermentation using the forage palm (Nopalea cochenillifera) as a substrate. For the optimization of incubation parameters, time, and temperature, the central composite rotational statistical model was used, resulting in an increase in enzymatic activity, by 94.70% with a maximum yield of 935.07 ± 21.70 IU/g at 23 °C and 56% moisture content. The β-glucosidase produced to show higher reactivity and stability at pH 5.0 at 52 °C. In addition to good halotolerance, maintaining 139.93% and 95.34% of the residual activity in the reaction and 57.93% and 94.28% after 24 h of incubation. The addition of Na⁺, Fe^{2 +}, Mg²⁺, EDTA, Triton X-100, lactose, and dichloromethane improved β-glucosidase activity, while Ca²⁺, Zn²⁺, and Co²⁺ were not expressed, and SDS, ethanol, acetone, and methanol were inhibitors. Therefore, it was possible to produce β-glucosidase with a halotolerant profile, indicating a promising alternative to obtain this enzyme for biotechnological and industrial applications.

这项研究首次调查了以饲草（Nopalea cochenillifera）为底物，通过固态发酵从罗氏青霉ATCC 10110生产β-葡萄糖苷酶。为了优化孵育参数，时间和温度，使用了中央复合旋转统计模型，从而使酶活性提高了94.70％，在23°C和56％时的最大产量为935.07±21.70 IU / g。水分含量。产生的β-葡萄糖苷酶在52°C的pH 5.0下显示出更高的反应性和稳定性。除具有良好的耐卤性外，在培养24小时后，还保留了反应中残留活性的139.93％和95.34％，以及在反应中保留的57.93％和94.28％。钠的添加⁺，铁^{2 +}，镁²⁺，EDTA，曲拉通X-100，乳糖，和二氯甲烷改善β葡糖苷酶活性，而钙²⁺，锌²⁺和Co ²⁺没有表达，及SDS，乙醇，丙酮和甲醇是抑制剂。因此，有可能产生具有卤代烷轮廓的β-葡糖苷酶，表明获得用于生物技术和工业应用的该酶的有前途的替代方法。