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Multiplexing cell-cell communication.
Molecular Systems Biology ( IF 9.9 ) Pub Date : 2020-07-16 , DOI: 10.15252/msb.20209618
John T Sexton 1 , Jeffrey J Tabor 1, 2
Affiliation  

The engineering of advanced multicellular behaviors, such as the programmed growth of biofilms or tissues, requires cells to communicate multiple aspects of physiological information. Unfortunately, few cell‐cell communication systems have been developed for synthetic biology. Here, we engineer a genetically encoded channel selector device that enables a single communication system to transmit two separate intercellular conversations. Our design comprises multiplexer and demultiplexer sub‐circuits constructed from a total of 12 CRISPR i‐based transcriptional logic gates, an acyl homoserine lactone‐based communication module, and three inducible promoters that enable small molecule control over the conversations. Experimentally parameterized mathematical models of the sub‐components predict the steady state and dynamical performance of the full system. Multiplexed cell‐cell communication has applications in synthetic development, metabolic engineering, and other areas requiring the coordination of multiple pathways among a community of cells.

中文翻译:

多路复用细胞间通信。

先进的多细胞行为的工程,例如生物膜或组织的程序化生长,需要细胞传达多个方面的生理信息。不幸的是,很少有细胞间通讯系统被开发用于合成生物学。在这里,我们设计了一种基因编码的通道选择器设备,使单个通信系统能够传输两个独立的细胞间对话。我们的设计包括由总共 12 个基于 CRISPR i 的转录逻辑门、基于酰基高丝氨酸内酯的通信模块和三个诱导型启动子构建的多路复用器和解复用器子电路,这些启动子能够实现小分子对对话的控制。子组件的实验参数化数学模型可以预测整个系统的稳态和动态性能。多重细胞间通讯可应用于合成开发、代谢工程和其他需要协调细胞群落中多种途径的领域。
更新日期:2020-08-01
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