Background. There is a pressing need for specific prognostic markers that could be used to monitor the severity of sepsis. The aims of our study were to investigate changes in the expression of different molecular forms of fibronectin in sepsis and to assess their relationship to the clinical severity and mortality of patients. Material and Methods. Forms of fibronectin: plasma (pFN), cellular (EDA-FN), FN-fibrin complexes, and fibronectin fragments were analyzed in 71 sepsis patients (survivors and nonsurvivors) and in the control by ELISA and immunoblotting. Results. The baseline pFN concentration of patients with sepsis was significantly lower than in the control (133.0 mg/L vs. 231.2 mg/L) (), and in nonsurvivors, it was lower than in survivors (106.0 mg/L vs. 152.8 mg/L) (). The baseline EDA-FN was significantly elevated in both sepsis groups (survivors: 6.7 mg/L; nonsurvivors: 9.4 mg/L) compared to the control (1.4 mg/L) (). It should be noted that among patients with more severe sepsis, the EDA-FN level was higher in nonsurvivors than in survivors. Furthermore, molecular FN-fibrin complexes as well as FN fragments occurred much more frequently in nonsurvivors than in survivors. Conclusion. The study showed that in sepsis, changes in plasmatic and cellular form of fibronectin were associated with the severity of sepsis and may be useful predictors of outcome.

中文翻译：

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血浆和细胞形式的纤连蛋白作为脓毒症的预后标志物。

背景。迫切需要可用于监测脓毒症严重程度的特定预后标志物。我们研究的目的是调查脓毒症中不同分子形式的纤连蛋白表达的变化，并评估它们与患者临床严重程度和死亡率的关系。材料和方法。纤连蛋白的形式：血浆 (pFN)、细胞 (EDA-FN)、FN-纤维蛋白复合物和纤连蛋白片段在 71 名脓毒症患者（幸存者和非幸存者）和对照中通过 ELISA 和免疫印迹进行了分析。结果。脓毒症患者的基线 pFN 浓度显着低于对照组（133.0 mg/L vs. 231.2 mg/L）（），在非幸存者中，它低于幸存者（106.0 mg/L vs. 152.8 mg/L）（）。与对照组（1.4 mg/L）相比，两个脓毒症组（幸存者：6.7 mg/L；非幸存者：9.4 mg/L）的基线 EDA-FN 均显着升高（）。应该注意的是，在更严重的脓毒症患者中，非幸存者的 EDA-FN 水平高于幸存者。此外，非幸存者比幸存者更频繁地发生分子 FN-纤维蛋白复合物以及 FN 片段。结论。该研究表明，在脓毒症中，纤连蛋白的血浆和细胞形式的变化与脓毒症的严重程度相关，并且可能是预后的有用预测因子。