The gynoecium is the most complex organ formed by the flowering plants. It encloses the ovules, provides a surface for pollen contact and self-incompatibility reactions, allows pollen tube growth and, post fertilization, and develops into the fruit. Consequently, the regulation of gynoecium morphogenesis is complex and appropriate timing of this process in part determines reproductive success. However, little is known about the global control of gynoecium development, even though many regulatory genes have been characterized. Here, we characterized dynamic gene expression changes using laser-microdissected gynoecium tissue from four developmental stages in Arabidopsis. We provide a high-resolution map of global expression dynamics during gynoecium morphogenesis and link these to the gynoecium interactome. We reveal groups of genes acting together early and others acting late in morphogenesis. Clustering of co-expressed genes enables comparisons between the leaf, shoot apex, and gynoecium transcriptomes allowing the dissection of common and distinct regulators. Furthermore, our results lead to the discovery of the LESSER FERTILITY1-4 (LEF1-4) genes, which, when mutated, lead to impaired gynoecium expansion, illustrating that global transcriptome analyses reveal yet unknown developmental regulators. Our data show that highly interacting proteins, such as SEPALLATA3, AGAMOUS, and TOPLESS are expressed more evenly during development, but switch interactors in time, whereas stage-specific proteins have only few interactors. Our analysis connects specific transcriptional regulator activities, protein interactions, and underlying metabolic processes towards the development of a dynamic network model for gynoecium development.

中文翻译：

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拟南芥（Arabidopsis thaliana gynoecium）形态发生过程中的高分辨率时空转录谱分析揭示了基因调控网络活动的年代，并揭示了新的发育调控因子。

妇科是开花植物形成的最复杂的器官。它包围胚珠，为花粉接触和自交不亲和反应提供表面，使花粉管生长并在受精后发展为果实。因此，妇科生殖器形态发生的调控是复杂的，该过程的适当时机部分决定了生殖的成功。然而，尽管已经对许多调节基因进行了表征，但对全球控制生殖生殖的知之甚少。在这里，我们表征了动态基因表达的变化，使用了来自拟南芥四个发育阶段的激光显微切割的妇科组织。我们提供了在生殖生殖形态发生过程中全球表达动态的高分辨率图，并将其链接到生殖生殖相互作用组。我们揭示了在形态发生中早期起作用且其他晚期起作用的基因组。共表达基因的聚类可以在叶，茎尖和妇科转录组之间进行比较，从而可以解剖共同的和不同的调节子。此外，我们的结果导致发现LESSER FERTILITY1-4（LEF1-4）基因，当该基因突变时，会导致妇科生殖细胞受损，说明全球转录组分析揭示了未知的发育调控因子。我们的数据表明，高度相互作用的蛋白（例如SEPALLATA3，AGAMOUS和TOPLESS）在发育过程中表达更为均匀，但会及时切换相互作用因子，而阶段特异性蛋白只有很少的相互作用因子。我们的分析将特定的转录调节因子活性，蛋白质相互作用，