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Isolation, identification and functional characterization of cultivable bacteria from Arabian Sea and Bay of Bengal water samples reveals high diversity
bioRxiv - Microbiology Pub Date : 2020-07-31 , DOI: 10.1101/2020.07.31.229039
Shriram N. Rajpathak , Yugandhara M. Patil , Roumik Banerjee , Asmita M. Khedkar , Pawan G. Mishra , Mandar Paingankar , Deepti D. Deobagkar

The oxygen minimum zone of the Arabian Sea (AS) and Bay of Bengal (BOB) is rich in organic matter and is an unusual niche. Bacteria present in the oceanic water play an important role in ecology since they are responsible for decomposing, mineralizing of organic matter and in elemental cycling like nitrogen, sulfur, phosphate. This study focuses on culturing bacteria from oxygen minimum zones (OMZ) and non-OMZ regions and their phylogenetic as well as the functional characterization. Genotypic characterization of the isolates using amplified rDNA based 16SrRNA sequencing grouped them into various phylogenetic groups such as alpha-proteobacteria, gamma-proteobacteria and unaffiliated bacteria. The cultivable bacterial assemblages encountered belonged to the genus Halomonas, Marinobacter, Idiomarina, Pshyctobacter and Pseudoalteromonas. Among the enzymatic activities, carbohydrate utilization activity was most predominant (100%) and microorganisms possessed amylase, cellulase, xylanase and chitinase. A large proportion of these bacteria (60%) were observed to be hydrocarbon consuming and many were resistant to ampicillin, chloramphenicol, kanamycin and streptomycin. The high diversity and high percentage of extracellular hydrolytic enzyme activities along with hydrocarbon degradation activity of the culturable bacteria reflects their important ecological role in oceanic biogeochemical cycling. Further assessment confirmed the presence of nitrogen reduction capability in these cultivable bacteria which highlights their importance in oceanic geochemical cycling.

中文翻译:

来自阿拉伯海和孟加拉湾水样的可培养细菌的分离,鉴定和功能表征显示高度多样性

阿拉伯海(AS)和孟加拉湾(BOB)的最低氧气区域富含有机物,是一个不寻常的生态位。海水中存在的细菌在生态中起着重要作用,因为它们负责分解,矿化有机物并参与元素循环(如氮,硫,磷酸盐)。这项研究的重点是从最小氧区(OMZ)和非OMZ区培养细菌及其系统发育和功能表征。使用基于扩增的基于rDNA的16SrRNA测序对分离株进行基因型鉴定,将其分为各种系统发育组,例如α-变形细菌,γ-变形细菌和非附属细菌。遇到的可培养细菌集合属于盐单胞菌属,马氏杆菌属,异狄氏杆菌属,假单胞菌属和假单胞菌属。在酶促活性中,碳水化合物利用活性最主要(100%),微生物具有淀粉酶,纤维素酶,木聚糖酶和几丁质酶。这些细菌中有很大一部分(60%)消耗碳氢化合物,并且许多细菌对氨苄西林,氯霉素,卡那霉素和链霉素具有抗性。可培养细菌的高多样性和高百分比的细胞外水解酶活性以及碳氢化合物的降解活性反映了它们在海洋生物地球化学循环中的重要生态作用。进一步的评估证实了这些可培养细菌中存在氮还原能力,这突出了它们在海洋地球化学循环中的重要性。纤维素酶,木聚糖酶和几丁质酶。这些细菌中有很大一部分(60%)消耗碳氢化合物,并且许多细菌对氨苄西林,氯霉素,卡那霉素和链霉素具有抗性。可培养细菌的高多样性和高百分比的细胞外水解酶活性以及碳氢化合物的降解活性反映了它们在海洋生物地球化学循环中的重要生态作用。进一步的评估证实了这些可培养细菌中存在氮还原能力,这突出了它们在海洋地球化学循环中的重要性。纤维素酶,木聚糖酶和几丁质酶。这些细菌中有很大一部分(60%)消耗碳氢化合物,许多细菌对氨苄西林,氯霉素,卡那霉素和链霉素具有抗性。可培养细菌的高多样性和高百分比的细胞外水解酶活性以及碳氢化合物的降解活性反映了它们在海洋生物地球化学循环中的重要生态作用。进一步的评估证实了这些可培养细菌中存在氮还原能力,这突出了它们在海洋地球化学循环中的重要性。可培养细菌的高多样性和高百分比的细胞外水解酶活性以及碳氢化合物的降解活性反映了它们在海洋生物地球化学循环中的重要生态作用。进一步的评估证实了这些可培养细菌中存在氮还原能力,这突出了它们在海洋地球化学循环中的重要性。可培养细菌的高多样性和高百分比的细胞外水解酶活性以及碳氢化合物的降解活性反映了它们在海洋生物地球化学循环中的重要生态作用。进一步的评估证实了这些可培养细菌中存在氮还原能力,这突出了它们在海洋地球化学循环中的重要性。
更新日期:2020-08-01
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