Manganese (Mn) plays an important role in the oxygen-evolving complex, where energy from light absorption is used for water splitting. Although changes in light intensity and Mn status can interfere with the functionality of the photosynthetic apparatus, the interaction between these two factors and the underlying mechanisms remain largely unknown. Here, maize seedlings were grown hydroponically and exposed to two different light intensities under Mn-sufficient or -deficient conditions. No visual Mn deficiency symptoms appeared even though the foliar Mn concentration in the Mn-deficient treatments was reduced to 2 µg g^–1. However, the maximum quantum yield efficiency of PSII and the net photosynthetic rate declined significantly, indicating latent Mn deficiency. The reduction in photosynthetic performance by Mn depletion was further aggravated when plants were exposed to high light intensity. Integrated transcriptomic and proteomic analyses showed that a considerable number of genes encoding proteins in the photosynthetic apparatus were only suppressed by a combination of Mn deficiency and high light, thus indicating interactions between changes in Mn nutritional status and light intensity. We conclude that high light intensity aggravates latent Mn deficiency in maize by interfering with the abundance of PSII proteins.

中文翻译：

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生理，转录组和蛋白质组学分析表明，高光强度加剧了玉米中潜在的锰缺乏症。

锰在放氧复合物中起着重要作用，在放氧复合物中，吸收光的能量用于分解水。尽管光强度和Mn状态的变化会干扰光合作用装置的功能，但是这两个因素之间的相互作用以及潜在的机理仍然很大程度上未知。在此，使玉米幼苗水培生长，并在锰充足或不足的条件下暴露于两种不同的光照强度。即使将缺锰处理中的叶面锰浓度降低至2 µg g ^–1，也没有出现可见的缺锰症状。。然而，PSII的最大量子产率效率和净光合速​​率显着下降，表明潜在的Mn缺乏。当植物暴露于高光强度时，由于Mn消耗而导致的光合性能的降低进一步加剧。综合的转录组学和蛋白质组学分析表明，光合作用中编码蛋白质的大量基因仅受Mn缺乏和强光的共同抑制，从而表明Mn营养状态和光照强度之间的相互作用。我们得出结论，高光强度会干扰PSII蛋白的丰度，从而加剧玉米中潜在的Mn缺乏症。