The potential of optical spectroscopic techniques such as diffused reflectance and fluorescence as non-invasive, in vivo diagnostic tools is being explored and validated recently. In this paper, we present the design and development of a handheld, portable, multimodal fiber optic based probe scheme to sequentially measure diffuse reflectance and fluorescence. The proposed prototype is designed to sequentially acquire diffused reflectance in the broad wavelength range of 400 nm-1600 nm and fluorescence using custom-chosen spectrophotometers, monochromatic and broadband light sources, fibers to accommodate a wide wavelength range, custom-built probe distal end, and a real-time spectral stitching and display unit. The prototype is characterized using in-house fabricated phantom tissue samples with tunable optical properties such as scattering and absorption. The depth profile study is carried out using phantom tissue layers of known optical parameters followed by the sequential measurement of diffused reflectance and fluorescence from the tissue mimicking sample.

中文翻译：

---

用于皮肤组织分析的便携式手持式多模态光谱探针系统的设计与开发

最近正在探索和验证光谱技术（如漫反射和荧光）作为非侵入性体内诊断工具的潜力。在本文中，我们介绍了一种基于手持式、便携式、多模态光纤的探头方案的设计和开发，以顺序测量漫反射和荧光。拟议的原型旨在使用定制选择的分光光度计、单色和宽带光源、光纤以适应宽波长范围、定制探针远端、在 400 nm-1600 nm 的宽波长范围内依次获取漫反射和荧光，和实时光谱拼接和显示单元。该原型使用内部制造的幻影组织样本进行表征，该样本具有可调谐的光学特性，例如散射和吸收。深度剖面研究是使用已知光学参数的幻影组织层进行的，然后依次测量来自组织模拟样本的漫反射和荧光。