We demonstrate the reconstruction of angle-resolved optical backscattering after transmission through a multimode fiber. Angle-resolved backscattering is an important tool for particle sizing, and has been developed as a diagnostic modality for detecting epithelial pre-cancer. In this work, we fully characterized the transfer function of a multimode fiber using a plane-wave illumination basis across two dimensions. Once characterized, angle-resolved scattering information, which has been scrambled by multimodal propagation can be easily and accurately reconstructed. Our technique was validated using a Mie theory-based inverse light scattering analysis algorithm on polystyrene microsphere phantoms of known sizes. To demonstrate the clinical potential of this approach, nuclear morphology was determined from the reconstructed angular backscattering from MCF-10A human mammary epithelial cell samples and validated against quantitative image analysis of fluorescence microscopy images.

中文翻译：

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通过用于细胞核和粒径测定的多模光纤重建角度分辨反向散射

我们展示了通过多模光纤传输后角分辨光学反向散射的重建。角度分辨后向散射是测量粒子大小的重要工具，并已发展成为检测上皮癌前病变的诊断方式。在这项工作中，我们使用二维平面波照明基础充分表征了多模光纤的传递函数。一旦表征，可以轻松准确地重建已被多模式传播扰乱的角度分辨散射信息。我们的技术使用基于 Mie 理论的逆光散射分析算法对已知大小的聚苯乙烯微球模型进行了验证。为了证明这种方法的临床潜力，