Ascocotyle longa is parasitic trematode with wide distribution throughout America, Europe, Africa, and Middle East. Despite the fact that this fish-borne pathogen has been considered an agent of human heterophyiasis in Brazil, the molecules involved in the host-parasite interaction remain unknown. The present study reports the proteome profile of A. longa metacercariae collected from the fish Mugil liza from Brazil. This infective stage for humans, mammals and birds was analyzed using nLC-MS/MS approach. We identified a large repertoire of proteins, which are mainly involved in energy metabolism and cell structure. Peptidases and immunogenic proteins were also identified, which might play roles in host-parasite interface. Our data provided unprecedented insights into the biology of A. longa and represent a first step to understand the natural host-parasite interaction. Moreover, as the first proteome characterized in this trematode, it will provide an important resource for future studies.

伞形长寿虫是寄生性吸虫，在美国，欧洲，非洲和中东地区分布广泛。尽管在巴西，这种鱼源性病原体被认为是人类异养症的病因，但宿主-寄生虫相互作用中涉及的分子仍然未知。本研究报告了从鱼类Migil liza收集的长。麦草的蛋白质组学概况来自巴西。使用nLC-MS / MS方法分析了这种对人类，哺乳动物和鸟类的感染阶段。我们鉴定到大量蛋白质，这些蛋白质主要参与能量代谢和细胞结构。还鉴定了肽酶和免疫原性蛋白，它们可能在宿主-寄生虫界面中起作用。我们的数据提供了对A. longa生物学的前所未有的见解，并代表了了解自然宿主与寄生虫相互作用的第一步。而且，作为这种吸虫的第一个蛋白质组，它将为将来的研究提供重要资源。